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交配型对香菇单、双核菌丝体菌丝生长速度的影响
  • ISSN号:1000-3924
  • 期刊名称:上海农业学报
  • 时间:2013
  • 页码:15-22
  • 分类:S646.1[农业科学—蔬菜学;农业科学—园艺学]
  • 作者机构:[1]国家食用菌工程技术研究中心,农业部南方食用菌资源利用重点实验室,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201403, [2]济宁学院食用菌研究所,济宁学院生命科学与工程系,曲阜273155, [3]上海海洋大学食品学院,上海201306
  • 相关基金:上海市科委重点科技攻关项目(10391900900);国家自然科学基金面上项目(31170098);山东省博士基金项目(2009BSA08009);山东省教育厅项目(J08LE51)资助
  • 相关项目:构建对应于香菇全基因组序列的连锁图谱及分析农艺性状QTL
中文摘要:

以野生香菇菌株HW21的120个单孢子分离物与4个遗传背景不同的其他香菇单核体杂交所得的480个双核体后代为材料,探讨了不同交配型对香菇单核体、双核体菌丝生长速度的影响。结果表明:不同交配型孢子单核体菌丝生长速度的变异系数为AxBx〈AxBy〈AyBx〈AyBy,菌丝生长速度为AxBxAxBy〉AyBx〉AyBy,其中AxBx和AyBy的菌丝生长速度差异极显著。含Ax因子的单核体菌丝速度大于含Ay因子的单核体,差异极显著;而含舭、却因子的单核体菌丝速度差异不显著。来自HW21的两个原生质体分离物菌丝生长速度为AxBx〉AyBy,差异极显著,推测交配型因子与控制单核体菌丝生长速度的基因位点之间有着某种连锁。PDA及木屑培养基中的单核体菌丝生长速度之间呈极显著遗传正相关,CYM及木屑培养基中双核体菌丝生长速度之间也呈极显著遗传正相关,但单核体与其杂交后代双核体菌丝生长速度之间无明显相关性。

英文摘要:

The 120 monospore isolates derived from wild L. edodes strain HW21 and their 480 dikaryotic hybrids obtained by crossing with 4 genetically different monokaryons were used as test ma- terials and the effects of different mating types on mycelial growth rates of the monokaryon and dikaryon mycelia were investigated. The results showed that the ascending order in variation coeffi- cients of mycelial growth rates of different mating types' monokaryons was AxBx 〈 AxBy〈 AyBx AyBy,the descending order in mycelial growth rates was AxBx〉 AxBy〉 AyBx〉 AyBy,and in myce- lial growth rate there was a very significant difference between AxBx and AyBy. The mycelial growth rates of monokaryons with Ax mating type factor were very significantly greater than ones with Ay factor,while there was not a significant difference between monokaryons with Bx and with By. And from the same trend and the same significance of difference existing in the two protoplast monokary- ons from HW21 ,it was inferred that there might be some linkage between the mating type factors and the gene loci controlling the mycelial growth rate. The positive correlation of the mycelial growth rates of monokaryons was very significant between on the PDA and on sawdust media,and that of the mycelial growth rates of the dikaryons was also very significant between on the CYM and on sawdust media,but there was not a clear correlation of mycelial growth rates between the monokaryons and the dikaryons.

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期刊信息
  • 《上海农业学报》
  • 北大核心期刊(2011版)
  • 主管单位:上海市农业委员会
  • 主办单位:上海市农业科学院
  • 主编:
  • 地址:上海市奉贤区金齐路1000号
  • 邮编:201403
  • 邮箱:xx6@saas.sh.cn
  • 电话:021-62202980 52235461
  • 国际标准刊号:ISSN:1000-3924
  • 国内统一刊号:ISSN:31-1405/S
  • 邮发代号:4-523
  • 获奖情况:
  • 荣获全国优秀科技期刊三等奖,中国农学会优秀农业期刊一等奖等.
  • 国内外数据库收录:
  • 英国农业与生物科学研究中心文摘,中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版)
  • 被引量:12545