中文摘要：

目的研究转化生长因子p诱导的基因（transforming growth factor-β induced gene，TGFBI）在体外是否能抑制恶性间皮瘤细胞NCI．H28的增殖。方法将外源性TGFBI稳定性转染到恶性间皮瘤细胞NCI—H28中，绘制生长曲线图，测定其细胞增殖、接种效率和软琼脂克隆形成，并进行细胞周期的分析。结果外源性TGFBI在恶性间皮瘤细胞NCI—H28中能够稳定地高表达；当外源性的TGFBI转入到肿瘤细胞NCI—H28后，TGFBI高表达的细胞倍增时间增加了4．38倍；与转染空载体的肿瘤细胞相比：NCI-H28的相对接种效率降低了69．68％，外源性TGFBI表达的T28细胞形成了较小的软琼脂克隆；TGFBI可以使肿瘤细胞阻滞在G1期，并延缓肿瘤细胞进入S期的时间。结论TGFBI可以抑制恶性间皮瘤细胞的体外增殖。

英文摘要：

Abstact： Objective To explore that transforming growth factor- 13 induced gene （TGFBI） inhibits NCI-H28 growth in vitro. Methods TGFBI was stably transfected into mesothelioma cell line NCI-H28. growth curve, cell proliferation, platting efficiency, and colony formation in soft agar were analyzed. Results Exogenous TGFBI in malignant mesothelioma NCI-H28 had a high expression in stably. After exogenous TGFBI transferred into tumor cells NCI-H28, doubling time of TGFBI high expression cell was increased by 4.38 times, compared with the empty vector. Relative plating efficiency of NCI-H28 was decreased by 69.68%. T28 cells with exogenous TGFBI formed a smaller soft agar; TGFBI made tumor cells arrest in Gl phase and delay tumor cells into the S phase .Conclusion TGFBI was able to inhibit the cell proliferation of malignant mesothelioma in vitro. Key words： Transforming growth factor-13 induced gene （TGFBI）; Malignant mesothelioma; Cell proliferation