中文摘要：

为探讨活性维生素D（1，25－dihydroxyvitamin D3，1，25（OH）2D3）与γ射线联合应用对SKOV－3细胞增殖的影响，评价1，25（OH）2D3能否增强γ射线对SKOV－3细胞的抑制作用，将实验分为1，25（OH），D，组，60Coγ射线组以及两者联合作用组和对照组，用MTT法测定细胞抑制率，并用流式细胞仪进行细胞周期、细胞内活性氧（ROS）和钙离子[Ca2＋]i分析。结果发现：1，25（OH）2D3与1，射线单独应用可以抑制SKOV－3细胞的增殖，并且1，25（OH）2D3能显著增强y射线对细胞的抑制作用；细胞周期分析显示，1，25（OH）2D3增加了SKOV．3细胞Go／G1期的比例，γ射线组增加了G2／M期的细胞比例，而联合作用组中G2／M期的比例明显增加，使细胞进一步阻滞于G2／M期；并且1，25（OH）2D3组和1，射线单独应用组均能增加细胞内ROS及[Ca2＋i]浓度，并且联合作用组增加幅度最大（P〈0．05）。结果提示，1,25（OH）2D3可以增强γ射线对卵巢癌细胞株SKOV－3抑制作用。

英文摘要：

To explore the combined effect of 1, 25-dihydroxyvitamin D3（1,25（OH）2D3） with 60Co γ-rays irradiation on the growth of ovarian cancer cell line SKOV-3, SKOV-3 cells were divided into 4 groups： 1, 25（OH）2D3 treatment group, irradiation group, combined treatment group and control group. Cell proliferation was determined by MTT. Distributions of cell cycle, ROS and [Ca2＋]i were analyzed by flow cytometry. The results showed that al- though each of two treatments alone displayed antiproliferative activity, the growth inhibition of SKOV-3 cells was further enhanced by the combination of 1, 25（OH）2D3 with irradiation. Cell cycle analysis indicated that the distribution of SKOV-3 cells in G0/G1 phase was significantly increased by 1, 25（OH）2D3, whereas, G2/M phase was further increased with its combination with irradiation. The results showed that the contents of ROS and [Ca2＋]i in the combined group cells were all significantly higher than that of the single treatment group （P〈0.05）. 1,25（OH）2D3 can enhance inhibition of ovarian cancer cells to γ-rays irradiation.