用盆栽法对小叶章不同生长阶段土壤CH4氧化能力随时间变化进行了研究.结果表明,氮输入后,植物各生长阶段的CH4氧化率均随时间推移发生了明显的波动变化.从生长季(6月7日~8月24日)CH4氧化率均值来看,只有12g/m^2处理对CH4氧化起促进作用,且12g/m^2和24g/m^2处理之间存在显著性差异(n=4,P〈0.05).不同氮输入水平对植物不同生长阶段CH4氧化率影响明显.第一阶段(6月7日~7月2日)适量(6g/m^2)氮输入对CH4氧化起促进作用,但过量(12g/m^2)氮输入则起抑制作用.第二阶段(7月2日~7月20日)只有12g/m^2处理起促进作用.第三(7月20日~8月7日)、四(8月7日~8月24日)阶段氮输入对CH4氧化起抑制作用.氮输入后,土壤微生物量碳(MBC)、土壤微生物量氮(MBN)、土壤基础呼吸(BR)、土壤代谢熵(qCO2)、土壤诱导呼吸(SIR)、铵态氮(NH4^+-N)、硝态氮(NO3^--N)和植物地上生物量与土壤CH4氧化的动态关系存在差异.土壤CH4氧化仅与MBC呈极显著负相关(P〈0.01),与其他影响因子相关性较小.
The abilities of CH_4 oxidation changing with time were studied by using pot-culture method at different growth stages of D.angustifolia.The time variation of CH_4 oxidation rate showed smart volatility after nitrogen input at each growth phase.From the perspective of the mean CH_4 oxidation rate of growing season(June 7~August 24),only the medial nitrogen level promoted CH_4 oxidation,and there existed significant differences between medial and high nitrogen level(n=4,P0.05).The effect of different nitrogen input level on the CH_4 oxidation rate was obvious at different growth stages.The first stage,moderate nitrogen input promoted CH_4 oxidation,but excessive nitrogen input suppressed CH_4 oxidation.The second phase,only medial nitrogen level promoted CH_4 oxidation.The third and fourth stages, nitrogen input both suppressed CH_4 oxidation.After nitrogen inputted,there were differences in dynamic relationships between soil microbial biomass C and N(MBC,MBN),soil basic respiration(BR),soil metabolic quotient(qCO_2),soil induced respiration(SIR),ammonium nitrogen(NH_4~+-N),nitrate nitrogen(NO_3~--N),plant biomass and CH_4 oxidation. Through correlative analysis,CH_4 oxidation was significantly related to MBC(P0.01).