中文摘要：

建立微乳液毛细管电动色谱法快速测定大鼠滑膜细胞内外液中雷公藤甲素含量的方法。在雷公藤甲素不同给药时间孵育条件下,将滑膜细胞分为空白对照组和给药组,测定滑膜细胞内外液中雷公藤甲素含量,并比较了细胞外液中雷公藤甲素在不同给药时间的含量变化。毛细管电泳运行缓冲液组成：1%（w/V）SDS,3%（V/V）正丁醇,1%（V/V）乙酸乙酯,96%（V/V）5 mmol/L硼砂70 mmol/L磷酸盐溶液,pH 9.0：运行电压：25 kV;压力进样：0.5 psi×6 s;电泳操作温度：25℃;检测波长：214 nm。结果表明,细胞内外液中的雷公藤甲素所呈现的线性关系良好相关系数分别为0.9995和0.9991。当给药24 h时,细胞内外液中的雷公藤甲素浓度分别为0.736和20.745μg/mL。本方法简单准确、灵敏度高、精密度好,可用于滑膜细胞内外液中雷公藤甲素浓度的动态变化规律研究,为进一步研究中药有效成分对滑膜细胞功能和活性的影响提供方法学基础。

英文摘要：

A method was established for the determination of triptolide in rat synoviocytes by microemulsion electrokinetic chromatography（MEEKC）.Synoviocytes were divided into control groups and treatment groups by the different administration time of triptolide,and the concentrations of triptolide in intercellular and extracellular fluid were determined.The determination of triptolide by MEEKC was performed at 25℃ under the conditions including running buffer of 5 mmol/L borate-10 mmol/L phosphate（96%,V/V,pH 9.0） with1%SDS（w/V）,3%1-butanol（V/V） and 1%ethyl acetate（V/V）,25 kV of running voltage,3.45 kPa×6 s of inject pressure and 214 nm of test wavelength.The results showed that triptolide was baseline separation,and there was a good linearity for triptolide in intercellular and extracellular of synoviocytes and the correlation coefficients were 0.9995 and 0.9991,respectively.The concentrations of triptolide in intercellular and extracellular of synoviocytes were 0.736 μg/mL and 20.745 μg/mL,respectively.The present method is simple,accurate,sensitive and precise,and can be used to study dynamic change of the triptolide concentration in rat synoviocytes and provide methodology basis for further study the influence of effective compounds of TCM on synoviocytes＇ function and activity.