中文摘要：

本文利用环糊精修饰毛细管胶束电动色谱法（CD-MEKC）同时分离检测橙皮苷和柚皮苷对映体。实验优化的条件为：以60mmol/L胆酸钠（SC）＋30mmol/L羟丙基-β-环糊精（HP-β-CD）＋20 mmol/L NaH2PO4-100 mmol/L NaOH（pH=9.0,97%（V/V））＋3%（V/V）甲醇为运行缓冲液,分离电压25kV,紫外检测波长214nm。在上述最佳条件下,橙皮苷和柚皮苷对映体在9min内得到完全分离,橙皮苷对映体的检测限（S/N=3）分别为0.13μg/mL和0.25μg/mL;柚皮苷对映体的检测限（S/N=3）分别为0.14μg/mL和0.07μg/mL。将所建立的方法用于胃苏颗粒制剂中橙皮苷和柚皮苷的对映体测定,回收率在86.0%～113.2%之间。

英文摘要：

In this study,an effective method of cyclodextrin modified chiral micelle electrokinetic capillary chromatography （CD-MEKC） was established to enantioseparate and detect hesperidin and naringin simultaneously. Under the following conditions：running buffer of 60 mmol/L SC＋30 mmol/L HP-β-CD ＋20 mmol/L Nail2 PO4-100 mmol/L NaOH（pH = 9. 0, 97G （V/V）） ＋3% （V/V） methanol, and separation voltage of 18 kV, the enantiomers were baselinly separated in 9 min. The detection limit （defined as S/N=3） of hesperidin and naringin enantiomers was ranged from 0.07 μg/mL to 0.25 μg/mL. The method can be successfully applied to the determination of hesperidin and naringin enantiomers in Weisu granules with satisfactory results.