中文摘要：

与把技术标记的荧光结合了，与飞米第二脉搏激光激动的二光子的显微镜学为神经科学研究成为了一个重要工具。在这研究，从在老鼠外皮片的神经原的钙信号被定做的二光子的显微镜学，和自发的钙信号和药理学回答第二象对飞米的回答一样监视脉搏激光刺激被记录。结果证明钙信号的振幅在直接比例增加了到相应电的活动。Glutamate 导致了钙短暂，却连续的申请导致了更小的反应。neuronal 人口的同时的监视区分了不同微型电路的神经原。飞米秒脉搏激光在金字塔形的神经原导致了本地或全球的钙信号。讯问的途径和用飞米第二脉搏激光的神经活动的控制是非接触，非破坏性，重复能，并且没有任何另外的底层，它将贡献神经科学的开发。

英文摘要：

Combined with the fluorescence labeling technique, two-photon microscopy excited with femtosecond pulse laser has become an important tool for neuroscience research. In this research, the calcium signals from neurons in rat cortex slice were monitored by a custom-built two-photon microscopy, and the spontaneous calcium signals and the pharmacological responses as well as the responses to femtosecond pulse laser stimulation were recorded. The results showed that the amplitude of the cal- cium signals increased in direct proportion to the corresponding electrical activities. Glutamate induced a calcium transient, but continuous application resulted in smaller response. Simultaneous monitoring of neuronal populations distinguished the neurons of different microcircuits. The femtosecond pulse laser induced local or global calcium signals in the pyramidal neurons. The approach of interrogation and control of neural activities using femtosecond pulse laser is non-contact, nondestructive, repeatable, and without any additional substrates, which will contribute to the development of neuroscience.