中文摘要：

现在的学习的目的是与不同剂量在照耀以后在 CD4+CD25+Nrp1+Treg 房间观察变化并且探索包含的可能的分子的机制的目的。方法 ICR 老鼠和老鼠淋巴瘤房间线(EL-4 房间) 被使用。CD4， CD25， Nrp1， calcineurin 和 PKC- 的表情被流动 cytometry 检测。TGF-1， IL-10， PKA 和营地的表情与 ELISA 被估计。在在照耀以后的 12 h 的结果， Nrp1 的表达式在 4.0 Gy 组显著地增加了，与假冒照耀的组相比(P < 0.05 ) 在怒气和胸腺，分别地当 ICR 鼠标收到了整个身体的照耀(WBI ) 时。同时， Interleukin 10 (IL-10 ) 和转变生长 factor-1 (TGF-1 ) 的合成在高剂量照耀(HDR ) 以后显著地增加了(> 或 = 1.0 Gy ) 。另外，，营地的表示和 PKA 蛋白质增加了 PKC- ， calcineurin 在 4.0 Gy X 照耀以后在胸腺房间在 12h 减少了。当当 PLC-PIP2 信号小径被刺激或 cAMP-PKA 信号小径在 4.0 Gy X 照耀以后被堵住时， TGF-1 清楚地被禁止时，这没在电离以后限制 CD4+CD25+Nrp1+Treg 房间的起来规定放射。这些结果显示了那 HDR 的结论可能导致 CD4+CD25+Nrp1+Treg 房间生产并且由在老鼠调整信号分子刺激 TGF-1 分泌物。

英文摘要：

Objective The purpose of the present study was to observe the changes in CD4＋CD25＋Nrpl＋Treg cells after irradiation with different doses and explore the possible molecular mechanisms involved. Methods ICR mice and mouse lymphoma cell line （EL-4 cells） was used. The expressions of CD4, CD25, Nrpl, calcineurin and PKC-α were detected by flow cytometry. The expressions of TGF-131, IL-10, PKA and cAMP were estimated with ELISA. Results At 12 h after irradiation, the expression of Nrpl increased significantly in 4.0 Gy group, compared with sham-irradiation group （P〈0.05） in the spleen and thymus, respectively, when ICR mice received whole-body irradiation （WBI）. Meanwhile the synthesis of Interleukin 10 （IL-20） and transforming growth factor-β1 （TGF-β1） increased significantly after high dose irradiation （HDR） （〉 or = 1.0 Gy）. In addition, the expression of cAMP and PKA protein increased, while PKC-α, calcineurin decreased at 12h in thymus cells after 4.0 Gy X-irradiation. While TGF-β1 was clearly inhibited when the PLC-PIP2 signal pathway was stimulated or the cAMP-PKA signal pathway was blocked after 4.0 Gy X-irradiation, this did not limit the up-regulation of CD4＋CD25＋Nrpl＋Treg cells after ionizing radiation. Conclusion These results indicated that HDR might induce CD4＋CD25＋Nrpl＋Treg cells production and stimulate TGF-β1 secretion by regulating signal molecules in mice.