中文摘要：

目的观察难治性颞叶癫痫患者脑组织内质网应激相关分子GRP78、caspase4的表达,探讨内质网应激在难治性颞叶癫痫脑损伤中的作用。方法应用免疫组化、Western blot方法检测32例难治性颞叶癫痫患者手术切除的颞叶皮层脑组织GRP78、caspase4的表达,并与性别、年龄相匹配的对照组进行比较。结果与对照组相比,GRP78、caspase4在癫痫组表达明显上调（P〈0.05）。结论癫痫诱发了内质网应激,caspase4可能参与了癫痫后脑损伤,很可能为癫痫脑损伤的治疗提供新的靶点。

英文摘要：

Objective To investigate the expression of GRP78 and caspase4,which are both endoplasmic reticulum stress associated molecules,in the resceted temporal cortex from patients with refractory temporal lobe epilepsy and explore the role of endoplasmic reticulum stress in the brain injury of human temporal lobe epilepsy.Methods The specimens were obtained from 32 patients with refractory temporal lobe epilepsy.Immunohistochemistry and western blot were used to determine the expression levels of GRP78 and caspase4,and compared to the controls with sex and age matched.Results GRP78 and caspase4 were obviously upregulated in the epilepsy brain if compared with the controls（P〈0.05）.Conclusion Chronic epilepsy induces endoplasmic reticulum stress and caspase4 may invlove in injury brain after repeated seizures,which may provide a new therapeutic target to prevent brain damage caused by eplilepsy.