中文摘要：

目的阐明狼（Canis lupus）和家犬（Canis familiaris）消化道内分泌细胞的形态、类型、局部分布和细胞密度的特点和异同。方法免疫组织化学技术链霉卵白素-过氧化物酶（SP）法。结果7种内分泌细胞在狼和家犬的消化道中均可检出。阳性细胞主要集中在狼和家犬胃腺、肠绒毛和肠腺上皮细胞间。某些部位的黏膜固有层和黏膜下层也有内分泌细胞分布。阳性细胞形态多样,多数有细胞突起伸向腺腔或肠腔,或伸向邻近细胞。在所检细胞中,以5-羟色氨（5-HT）阳性细胞的分布最为广泛,其次为血管活性肠肽（VIP）阳性细胞。其余5种细胞的分布具有一定的区域性。比较狼和家犬的检测结果发现,除胰多肽（PP）阳性细胞和P物质（SP）阳性细胞的分布位置和某些内分泌细胞的分布密度存在较大差别外,大多数消化道内分泌细胞具有相同的分布特征。结论狼和家犬的消化道内分泌细胞具有哺乳动物的一般特征。两者消化道内分泌细胞的形态和分布的相似性证明了犬科动物在消化生理结构上的一致性;同时,狼的消化道内分泌细胞又体现出了野生哺乳动物的独有特征。

英文摘要：

Objective To clarify the morphological features, types, regional distributions and cell densities of gastrointestinal endocrine cells in various parts of the digestive tracts of Canis lupus and Canis familiaris. Methods Immunohistochemical techniques of streptavidin- peroxidaso （SP） method was used. Results Seven kinds of gastrointestinal endocrine cells were found positive in the digestive tracts of Canis lupus and Canis familiaris. The endocrine cells were mainly located between the epithelia cells of gastric gland, intestinal villus and intestinal gland. There were also some endocrine cells distributed in the lamina propria and submucosa. The endocrine cells found in the digestive tract were in various shapes. Most of the endocrine cells had long cell processes run ning out to the lumen or the nearby cells. Among these endocrine cells, 5- hydroxytryptamine （5-HT） positive cells had a widest distribution, followed by vasointestinal poplypetide（VIP） positive cells. The distributions of other cells were limited. From the comparison of the characters of endocrine cells of Canis lupus and Canis familiaris, most of the endocrine cells had similar morphological features and regional distributions except pancreatic polypeptide （PP） positive cells and substance P （SP） positive cells. There were differences in cell densities of some endocrine cells. Conclusion The results of Canis lupus and Canis familiaris reflect the similarity of mammals in the characteristics of the morphological features, types, regional distributions and cell densities of gastrointestinal endocrine cells, as well as the similarity in digestive physiology of canine animals. At the same time, the results of Canis lupus also exhibit characteristics of gastrointestinal endocrine cells of wild animals.