中文摘要：

利用EST随机挑取克隆测序的方法从盐地碱蓬叶片cDNA文库中分离得到了盐地碱蓬V-H^＋ -ATPaseH亚基cDNA序列，并进行了H、c亚基基因表达及V-H^＋ -ATPase活性分析。结果表明，H亚基基因全长1969bp，包括100bp的5＇-非编码区和471hp的3＇-非编码区。开放阅读框为1398bp，编码465个氨基酸残基，分子量约52．8kD。Northern杂交分析表明盐胁迫明显诱导了H亚基表达，而且盐胁迫下H、c亚基及V-H^＋ -ATPase活性存在协同作用。这些结果表明盐胁迫下H和c亚基基因上调及V-H^＋ -ATPase活性的增加为Na^＋区隔化到液泡中提供了质子驱动力。

英文摘要：

The cDNA sequence of subunit H of V-H^＋-ATPase was isolated by EST random cloning and sequencing from cDNA library of Suaeda salsa leaves and then the expressions of ssVHA-H and ssVHA-c and the activity of V-H^＋-ATPase were analyzed. The results showed that the full length of H-subunit was 1 969 bp,including 100 bp 5＇-noncoding region and 471 bp 3＇-coding region. The open reading frame was 1398 bp,encoding 465 amino acid residues and having a molecular weight of 52.8 kD. Salt stress was found capable of significantly inducing subunit H expression,and ssVHA-H,and ssVHA-c and the activity of V-H^＋-ATPase appeared to act in a synergistic manner under the stress. These indicated that under salt stress the expression up-regulations of ssVHA-H and ssVHA-c genes and the increase of the activity of V-H^＋-ATPase provide the proton driving force for sequestrating Na^＋ in vacuoles.