中文摘要：

以吡咯为前驱体,羧基化碳纳米管、石墨粉为填料和碳酸钙微球为模板直接诱导合成,制备出一种高灵敏的多孔＂类碳糊电极＂生物电化学传感器,讨论了羧基化碳纳米管含量、银染时间对检测结果的影响。结果表明,最佳羧基化碳纳米管含量为8%,最佳银染时间为12min,银的阳极溶出峰电流与E.ocliO157：H7浓度在1.0×104～1.0×106cells/mL范围内呈线性关系,其线性回归方程为：IP=-5.582＋1.972logC,相关系数（R2）为0.9912,检出限为5.1×103cells/mL,实现了对E.ocliO157：H7快速、准确地检测。

英文摘要：

A novel and sensitive electrochemical biosensor based on porous pseudo-carbon paste electrode（PPCPE） was described.PPCPE was fabricated by mixing calcium carbonate microspheres as the template,graphite powders and carbon nanotubes as the fillers,pyrrole as the precursor of polymer which actually acted as the pastE.The effects of content of carbon nanotube functionalized with carboxyl group（NWCNT-COOH） in carboxylated of porous pseudo-carbon paste electrode（PPCPE-COOH） and the silver enhancement time were investigated.The results showed that the optimal contents of NWCNT-COOH was 8%,the optimal silver enhancement time was 12 min.A linear relationship between the silver anodic stripping peak current and the concentration logarithm of E.ocli O157：H7 from 1.0×104 to 1.0×106 cells/mL was obtained,with a correlation coefficient of 0.9912 and a detection limit of 5.1×103 cells/mL.