中文摘要：

以膦酰基羧酸共聚物为修饰剂,采用水热法制备了LaPO4∶Ce,Tb发光纳米粒子,该粒子具有良好的水溶性和生物相容性。对合成的纳米粒子进行了发光光谱、X-射线衍射、透射电子显微镜和红外光谱表征。以制备的纳米粒子为能量供体,纳米金为能量受体,细胞色素C（Cyt C）为桥,构建了LaPO4∶Ce,Tb-Au发光共振能量转移（LRET）体系,据此建立了测定Cyt C的新方法。实验结果表明,当Cyt C的浓度在0.33321.0μg·mL-1范围时,LRET体系发光猝灭程度与Cyt C浓度呈良好线性关系,检出限（S/N=3）为0.2μg·mL-1,相对标准偏差（RSD）为0.46%（c=10.0μg·mL-1,n=11）。该方法用于实际样品的测定,回收率范围为100%101%。

英文摘要：

Copolymer of phosphono and carboxylic acid（POCA）modified LaPO4∶Ce,Tb nanoparticles（LaPO4∶Ce,Tb NPs）with good water-solubility were synthesized by hydrothermal method,and characterized by luminescence spectrum,X-ray diffraction（XRD）,transmission electron microscopy（TEM）and Fourier transform infrared（FT-IR）spectrometer.In this paper,a luminescence resonance energy transfer（LRET）system using the synthesized LaPO4∶Ce,Tb NPs and gold nanoparticles（Au NPs）as the energy donor and acceptor,respectively,and the cytochrome C（Cyt C）as bridge,was constructed for the determination of Cyt C.As a result,the quenching of the luminescence intensity in the LRET syetem was linearly correlated to the concentration of Cyt C in the range of 0.333-21.0μg·mL-1.Such bridge-type LRET-based approach can reach a detection limit of 0.2μg·mL-1（S/N=3）with relative standard deviation（RSD） of 0.46%（c=10.0μg·mL-1,n=11）.The proposed method was successfully applied in the determination of Cyt C in samples,with the recoveries of 100%-101%.