作者于2007和2008年的5-10月间在辽宁省的丹东、凤城、清原、桓仁等药材产区采集土壤样品200余份,通过采用稀释平板法和土壤颗粒平板法进行土壤真菌的分离和培养,共分离和鉴定出27属54种真菌,其中接合菌2属3种、子囊菌1属1种、无性型真菌24属50种。分离到的部分真菌种类是重要的药用植物病原菌,如Cylindrocarpon destructans、Fusarium oxysporum和F.solani。研究发现,Penicillium paxilli、P.expansum、Trichoderma atroviride及T.viride的分离频率最高,是辽宁省4个调查地区药用植物根际土壤真菌的优势菌群。
During May and October in 2007 and 2008, more than 200 soil samples were collected from the rhizosphere of medicinal plants in Liaoning Province. The sampling sites include Dandong, Fengcheng, Qingyuan and Huanren. Soil diluting plate and soil particle plate methods were used to isolate the fungi. As a result, 54 fungal species belonging to 27 genera were obtained. Among them, three species in two genera belong to zygomycetes, one species belongs to ascomycetes, while 50 species in 24 genera areanamorphic fungi. Some of them are important pathogens of medicinal plants, such as Cylindrocarpon destructans, Fusarium oxysporum and F. solani. Penicillium paxilli, P. expansum, Trichoderma atroviride and T. viride are predominant groups of fungi in the rhizosphere soil of medicinal plants in Liaoning Province.