在采用不同药剂处理破除嵩草种子休眠的基础上,利用高效液相色谱测定嵩草种子中脱落酸含量变化,探讨影响嵩草种子休眠的因素。结果表明:40%的NaOH处理使矮生嵩草及高山嵩草种子的发芽率达到最高,分别为56.00%和46.00%。处理前种子中的脱落酸含量分别为3.451和4.571μg/g,处理后脱落酸含量分别为1.225和1.813μ/g,分别下降了64.50%和60.34%。
Germination tests have been carried out with different treatments on the seeds of Kobresia Willd and the quantity of abscisic acid before and after the treatment was determined by a HPLC method. The results showed that the germination rates of both Kobresia humilis and Kobresia pygrnaea reached the highest with a treatment of 40% NaOH, which was separately 56. 00% and 46.00%. The abscisic acid in the seeds was separately 3. 451 μg/g and 4. 571 μg/g before the treatment and after the treatment was separately 1. 225 μg/g and 1. 813 μg/g, which decreased separately by 64.50% and 60.34%.