中文摘要：

基于微孢子虫分类学地位的争议，通过克隆家蚕微孢子虫（Nosema bornbycis）的α-Tubulin基因，利用BioEditor将核苷酸序列翻译成氨基酸序列，并从NCBI中收集不同物种的仅-Tubulin基因，用CLUSTALX（1．81）、Mega2以及在线生物信息学软件CLUSTALW（1．83）分析序列并构建系统发育树。结果显示：家蚕微孢子虫及其它微孢子虫与真菌聚为一类，且微孢子虫以一个独立群与接合菌（zygomycetes）的噬虫霉属（Entomophaga）、耳霉属（Conidiobolus）关系最近，与子囊菌（ascomycetes）、担子菌（basidiomycetes）、壶菌（chytrids）及其它接合菌互为姐妹群。

英文摘要：

The taxonomy position of microsporidia has caused intense argument in the world. A partial sequence of α-Tubulin gene of Nosema bombycis was cloned and translated to amino acids by BioEditor software. Several α-Tubulin genes of different species were collected from Genbank of NCBI. By using ClustalX （1.81）, Mega2 software and online ClustalW（1.83） two phylogenetic trees were constructed. The result indicated that microsporidia was a member of fungi group. As a single sub-group microsporidia showed the nearest relationship with Entomophaga and Conidiobolus which were zygomycetes, and as a sister group to ascomycetes, basidiomycetes, chytrids and other zygomycetes.