中文摘要：

通过在碳纳米管修饰玻碳电极表面电聚合的方法制备了聚对氨基苯磺酸/碳纳米管复合膜修饰电极（PABSA/CNT/GC）,采用扫描电镜对电极形貌进行了表征。运用循环伏安法研究了尿酸（UA）和抗坏血酸（AA）在该修饰电极上的电化学行为,在pH7.0的PBS中,UA和AA分别在0.312、-0.025 V处产生灵敏氧化峰,与其在聚氨基苯磺酸和碳纳米管单层膜修饰电极上的电化学行为相比,两者的氧化峰电流显著增加,峰电位差（ΔEpa）达到337 mV,表明碳纳米管和聚合物产生协同增效作用,探讨了其作用机理。在优化实验条件下,建立了差分脉冲伏安法同时测定UA和AA的方法,UA、AA的线性范围分别为2.5×10-7~5.0×10-4、8.0×10-6~4.0×10-3mol/L,检出限分别为7.5×10-8、5.0×10-6mol/L。该方法用于尿样中UA和AA的测定,结果令人满意。

英文摘要：

A poly（p-aminobenzene sulfonic acid）/carbon nanotube composite-modified glassy carbon electrode（PABSA/CNT/GC）was fabricated by electropolymerizing p-aminobenzene sulfonic acid on the surface of glassy carbon electrode modified with carbon nanotube.The morphology of the electrode surface was characterized by scan electron microscopy（SEM）.The electrochemical behaviors of uric acid（UA） and ascorbic acid（AA） were investigated using cyclic voltammetry（CV） at the prepared electrode.In pH 7.0 PBS,UA and AA generated a sensitive anodic peak at 0.312 V and-0.025 V,respectively.Comparing with PABSA or CNT single layer modified electrodes,the composite-modified electrode had superior electrocatalytic activity.A dramatic enhancement of the peak current and a peak to peak separation of 337 mV was observed,which indicated that a synergistic effect was existed between carbon nanotube and polymer.The further reaction mechanism was discussed.Differential pulse voltammetry（DPV） was used for the simultaneous detecting UA and AA under the selected conditions.The linear range was 2.5×10-7-5.0×10-4 mol/L with a detection limit of 7.5×10-8 mol/L for UA and 8.0×10-6-4.0×10-3 mol/L with a detection limit of 5.0×10-6 mol/L for AA.The PABSA/CNT/GC electrode showed good stability,high selectivity and wide linear range.The proposed method was further used to determine UA and AA in human urine with satisfactory result.