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CdSe量子点荧光增敏法测定甘草酸

ISSN号：1004-4957
期刊名称：分析测试学报
时间：2011.11.11
页码：1241-1245
分类：O561.3[理学—原子与分子物理;理学—物理] O611.63[理学—无机化学;理学—化学]
作者机构：[1]青岛大学纤维新材料与现代纺织实验室国家重点实验室培育基地化学化工与环境学院,山东青岛266071
相关基金：国家自然科学基金项目（20975056,50802045,81102411）;青岛市科技计划基础研究项目（091330jeh）;NSFC-JSPS中日合作与交流项目（21111140014）;山东省自然科学基金（ZR2011BZ004,ZR2011BQ005）;山东省泰山学者项目（TS20070711）
相关项目：基于碳纳米管/量子点纳米纤维的微型电化学生物传感器的制备及其在蛋白质检测中的应用

作者：高艳丽|王宗花|张菲菲|夏建飞|夏延致|李延辉|GAO Yan-li,WANG Zong-hua*,ZHANG Fei-fei,XIA Jian-f|

关键词： CDSE量子点, 甘草酸, 荧光增敏法, CdSe quantum dots, glycyrrhizin, fluorescence enhancement

中文摘要：

以巯基乙酸为稳定剂水相法一步合成了CdSe量子点，并以此量子点为荧光探针，基于甘草酸（GL）对量子点的荧光增强效应，建立了一种简便、快速、灵敏测定GL的分析方法，同时对GL与CdSe量子点的反应机理进行了初步探讨。考察了多种因素对GL测定的影响。结果表明，在pH7．4的Na2HPO4-NaH2PO4缓冲溶液中，当量子点的浓度为2．4×10～mol／L、反应时间为10min时，体系的相对荧光强度与GL的质量浓度呈良好线性，线性范围为5．0×10^-91．0×10^-7g／L，r=0．9980，检出限为4．8×10^-9g／L。该方法对实际样品中甘草酸的测定结果与标准药典方法相吻合。该研究结果预示量子点修饰的甘草酸靶向药物运载系统具有较好的示踪效果，对进一步研究甘草酸靶向运载体系具有一定意义。

英文摘要：

Water-soluble CdSe quantum dots （QDs） were synthesized in aqueous solution with thioglycollie acid as stabilizer. By using CdSe QDs as fluorescent probe, based on the enhancement of the fluorescence intensity of CdSe QDs by glycyrrhizin （GL）, a simple, rapid and sensitive method for the determination of GL was proposed and validated. Forthermore, the possible mechanism of interaction between CdSe QDs and GL was discussed. Effects of experimental conditions were investigated. The optimal conditions were as follows ： buffer： pH 7.4 Na2HPO4 - Nail2PQ solution, concentration of quantum dots： 2.4 × 10^-4 mol/L, reaction time： 10 min. Under the optimal condi- tions, a good linearity was obtained between fluorescence intensity and GL concentration in the range of 5.0 × 10^ -9 - 1.0 × 10 ^-7 g/L with a correlation coefficient of 0. 998 0. The obtained linear regression equation was： IF/IF0 = 1. 337 ＋ 0. 014p（ 10^-9 g/L） and the limit of detection was 4. 8 × 10^-9 g/L. The proposed method was successfully applied in the determination of GL in actual samples, and the results were in good agreement with that of the national standard method in Chinese Pharmacopoeia. The study indicated that the combination of QDs and GL produced a nice labelling effect, and it would be of considerable significance to the further investigation of GL targeted carrier system.

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