中文摘要：

目的 建立同时测定萝芙木中的5种吲哚类生物碱含量的方法。方法 采用Diamonsil C_（18）色谱柱（250 mm×4.6 mm,5μm）,柱温25℃,流动相为甲醇-水（梯度洗脱）,流速1 m L·min^-1,检测波长280 nm,进样量5μL。结果 萝芙木中蛇根精、育亨宾、萝芙木碱、阿马里新、利血平5种吲哚类生物碱在1.56~200.0μg·m L^-1范围内线性关系良好,相关系数均大于0.999 0,5种吲哚类生物碱的平均回收率为95.0%~105.0%（n=9）。结论 该方法简单,结果准确、重复性好,可用于萝芙木药材的质量控制。

英文摘要：

Objective To establish an high perfermance liquid chromatography method for determination of five indole alkaloids in Rauvolfia. Methods The Diamonsil C18 column was used at 25 with methanol-water （gradient elution） as the mobile phase, the flow rate was 1. 0 mL＊min 1,the detective wavelength was 280 nm, and the injection volume was 5 ml·L^-1. Results In the range of 1. 56 - 200 μg·m L^-1, the correlation coefficients of regression equations of sarpagine, yohimbine, ajmaline, ajmalicine, reserpine were higher than 0. 999 0. The average recovery （ n = 9 ） of five indole alkaloids was between 95. 0% - 105. 0%. Conclusion This method is simple with good accuracy and repeatability. It can be used for quality control of Rauvolfia.