中文摘要：

目的 通过miRNA芯片谱研究,分析FlaA N/C蛋白辐射防护功能的转录后调控机制.方法 FlaA N/C蛋白皮下注射小鼠2h后,取小肠组织进行miRNA芯片谱分析,芯片谱结果用生物信息学方法预测miRNA作用靶点及可能相关的信号通路.结果 FlaA N/C皮下注射后2h有108个miRNA上调,59个miRNA下调,差异miRNA预测靶点主要集中在NFκB、Pl3K-Akt、p53和Caspase等信号通路.结论 凋亡相关信号通路在FlaA N/C介导辐射防护机制中具有重要作用,有必要针对凋亡相关miRNAs网络开展辐射防护机制研究.

英文摘要：

Objective To analysize the post-transcriptional regulation mechanism of FlaA N/C protein,chip spectrum of miRNA were studied. Methods Intestinal tissue were collected for miRNA chip spectrum analysis 2 hours after subcutaneously injection of new recombinant FIaA N/C protein in miee. Subsequently, bioinformatics were applied to forecast the target proteins and related signaling pathways. Results 108 miRNAs were up-regulated and 59 miRNAs down-regulated. The target proteins were mainly distributed in NF-kB,PI3K Akt,p53,and Caspase pathways. Conclusion Apoptosis related signal pathway plays an important role in the mechanisms of radioprotection. Further study should focus on apoptosis related miRNAs network.