中文摘要：

目的探讨以嗜肺军团菌LPl株MompS抗原表位为基础的合成肽的免疫作用。方法在GenBank中检索LPl株MompS基因信息，利用在线生物信息学软件，结合DNAStar软件的蛋白质二级结构预测理论方案，预测其B表位抗原位点，设计合成具有LPl血清型特异性氨基酸序列的多肽，通过肽结合实验和动物免疫学效果评价检测合成肽对LPl免疫血清的结合能力。结果筛选得到3条多肽GP-15、NY-17、AA-19，多肽肽结合实验表明GP-150（450）反应值显著高于NY．17和AA-19片段（P〈0．05），动物免疫学效果评价显示其各时间段的IgG抗体几何平均滴度显著高于NY-17和AA-19（P〈0．05）。结论证实3条多肽均具备了MompS表位属性，GP-15肽结合实验和动物免疫效果评价均提示其可作为嗜肺军团菌多肽疫苗的候选肽段。

英文摘要：

Objective To explore the immune function of antigen epitope peptides based on MompS antigen of Legionella pneumophila serogroup 1 （ LP1 ）. Methods The genetic information of LP1 MompS antigen was retrieved in GenBank by using bioinformatics software online. Combined with the prediction scheme theory of protein secondary structure by DNA Star, the B epitope antigen sites were predicted, and then the polypeptides which have the specific amino acid sequence of LP1 were synthesized. The binding ability of the obtained polypeptides to LP1 was detected by peptides binding assay and animal immunology effect assessment. Results Three obtained peptides, GP-15, NY-17 and AA-19 were screened out, and the GP-15 peptide had the best immunogenic ability among 3 peptides in the peptides binding assay （ P 〈 0. 05 ） and animal immunology effect assessment （P 〈 0. 05 ）. Conclusion All of the 3 peptides are MompS antigen epitope peptides, and the GP-15 can be used to construct vaccine of Legionella pneumophila.