中文摘要：

目的：观察siRNA敲低S100A4的表达后对胶质瘤细胞系SNB19侵袭和迁移能力的影响。方法S100A4干扰RNA（siRNA）敲低SNB19细胞中S100A4的表达（n=3），同时设control组（空白对照组，n=3）和siR-NA-NC组（阴性对照组，n=3），采用RT-PCR和western blot方法分别检测S100A4被有效敲低，划痕实验和Tran-swell实验分别检测细胞的迁移和侵袭能力的变化，western blot法检测基质金属蛋白酶2（MMP-2）、基质金属蛋白酶9（MMP-9）和E-cadherin的表达变化，倒置相差显微镜观察细胞片状伪足变化情况。结果 siRNA技术可显著下调SNB19细胞中S100A4 mRNA和蛋白的表达水平，siRNA-NC组和siRNA-S100A4组的mRNA较control组的表达量分别是0.97±0.07和0.21±0.04（P〈0.01），三个组的蛋白相对表达量分别为78.12%±2.63%、77.16%±3.00%和37.95%±2.71%（P〈0.01）；干扰成功后，siRNA-S100A4组与control组相比，细胞的迁移和侵袭能力分别降低了46%和55%，MMP-9和MMP-2的蛋白表达水平分别下调了62%和68%，E-cadherin的表达水平上调了154%，细胞片状伪足较对照SNB19细胞相比明显变小，差异均有统计学意义（P〈0.01）。结论敲低S100A4表达可降低胶质瘤细胞系SNB19的侵袭和迁移能力，提示S100A4可能成为抗胶质瘤侵袭迁移治疗的有效靶点。

英文摘要：

Objective To investigate the effects of siRNA-mediated knockdown of S100A4 expression on the inva-sion and migration of SNB19 glioma cells. Methods The S100A4 expression was knockdowned using S100A4 siRNA in SNB19 glioma cells. Glioma cells were assigned into control group,siRNA-negative control treated group （siRNA-NC） and siRNA-S100A4 group. RT-PCR and western blot were used to detect the mRNA and protein expression of S100A4, respectively. The wound-healing assay and transwell invasion assay were used to determine the ability of migration and invasion of SNB19 glioma cells, respectively. The expression of matrix metalloproteinase 9 （MMP-9）, matrix metallopro？teinase 2 （MMP-2） and E-cadherin proteins were evaluated by using western blot. Moreover, the morphology of lamellipo？dia of glioma cells were examined by using inverted phase-contrast microscopy. Results The mRNA and protein expres-sion levels of S100A4 was obviously down-regulated after transfection of S100A4 siRNA. Compared with control group, the mRNA expression levels of S100A4 in siRNA-NC group and siRNA-S100A4 group were 0.9±0.07 and 0.21±0.04,respectively（P〈0.01）. The protein expression levels of S100A4 in control, siRNA-NC and siRNA-S100A4 groups were 78.12%±2.63%, 77.16%±3.00%and 37.95%±2.71%, respectively（P〈0.01）. The migration and invasiveness capability were decreased up to 46% and 55% in the siRNA-S100A4 group compared with the control group（P〈0.01）. The pro？tein expression levels of MMP-9 and MMP-2 were inhibited up to 62% and 68%（P〈0.01）whereas the expression of E-cadherin was increased up to 154%（P〈0.01）in the siRNA-S100A4 group. The lamellipodia became smaller or unex？tended in siRNA-S100A4-treated SNB19 glioma cells. Conclusion S100A4 plays an important role in the invasion and migration of glioma cells, suggesting that S100A4 might be a potential candidate for anti-glioma strategy to prevent the invasion and migration of glioma cells.