中文摘要：

目的研究大豆异黄酮对新生儿期雌性大鼠卵巢发育的影响。方法将孕鼠随机分至大豆异黄酮0、10、50、100、150和200mg/kg bw染毒剂量组中,在母鼠产后第5~10天通过给母鼠灌胃使新生儿期大鼠间接暴露大豆异黄酮,在产后第11天分离子代雌性大鼠的卵巢进行病理检查,并用免疫组织化学和RT-PCR方法测定卵巢中雌激素受体（ER）、雄激素受体（AR）、孕激素受体（PR）和增殖细胞核抗原（PCNA）及其mRNA的表达水平。结果与对照组比较,大豆异黄酮100、150和200mg/kg bw剂量组的卵巢/体重显著增加,卵泡发育加快;150mg/kg bw和200mg/kg bw剂量组卵巢ER及其mRNA和PCNA蛋白表达显著增加,而PR mRNA表达显著降低;但卵巢AR、PR蛋白及AR mRNA的表达各处理组与对照组差异无显著性。结论新生儿期暴露大豆异黄酮的雌性大鼠卵巢发育表现出明显的不良作用,这种作用至少涉及到雌激素敏感受体ER、PRmRNA的转录。

英文摘要：

Objective To study the effects of lactational exposure to soy isoflavones （SIF） on ovary development in neonate rats. Methods Virgin Sprague-Dawley female and male rats were fed with soy and alfalfa free diets （SAFD）. The pregnant rats were randomly divided into six groups with seven animals in each group. Dams in different treatment groups received 0, 10, 50, 100, 150 and 200mg/kg bw of SIF from postnatal day 5 to 10 （PNDS-10） by gavage, respectively. On PND11, female litters were killed and ovaries were removed for pathological evaluation and detection of estrogen receptor（ER）, andron receptor （AR）, progesterone receptor （PR）, proliferating cell nuclear antigen （PCNA） protein expression by immunohisiochemistry as well as ER, AR, PR mRNA expression by RT-PCR, Results The ratios of ovary weight to body weight increased significantly in 100, 150 and 200mg/kg bw groups than that in control group. And the ovary follicles developed more quickly in offsprings exposed to SIF. In 150 and 200mg/kg bw groups, ER, PCNA protein and ER mRNA expression in ovaries increased significantly, while PR mRNA expression decreased significantly. Other receptor expressions had no significant difference from the control group, Conclusion Lactational exposure to soy isoflavones could induce adverse effects on ovary development in neonate rats, which mechanisms may, at least, partically involved with modification of mRNA transcription for ER and PR.