目的探讨推拿手法联合跑台训练对坐骨神经横断伤外膜修复后促进神经再生的效应及途径。方法 96只Sprague-Dawley大鼠随机分为正常对照组、缝合对照组和缝合手法组,每组32只。后两组建立坐骨神经横断伤外膜缝合模型,缝合手法组给予捻揉手法及跑台训练,每天1次。分别于干预后2、3、4、8周各组取8只大鼠检测坐骨神经传导速度、再生神经轴突数及施万细胞数。结果与缝合对照组比较,缝合手法组坐骨神经传导速度在干预后4周、8周加快(P〈0.05),轴突数目在2周、4周时有显著性差异(P〈0.05);施万细胞数无显著性差异(P〉0.05)。结论推拿手法联合跑台训练可促进损伤的周围神经再生。
Objective To explore the effect of Tuina combined with treadmill training on nerve regeneration after sciatic nerve transection. Methods Ninty-six Sprague-Dawley rats were randomly divided into normal group(n=32), model group(n=32) and treatment group(n=32). The sciatic nerve was transected and the epineurium was sutured in the latter two groups. The treatment group was given Tuina manipulation and treadmill training once a day. Eight rats from each group were detected their sciatic nerve conduction velocity(NCV), the number of axons and Schwann cells(SCs) two, three, four and eight weeks after intervention. Results Compared with the model group, the NCV accelerated in the treatment group four and eight weeks after intervention(P〈0.05); while the number of axons was significantly different two and four weeks after intervention(P〈0.05), and the number of SCs was not very different between the treatment group and the model group after intervention(P〉0.05). Conclusion Tuina combined with treadmill training may promote the regeneration of peripheral nerve after injury in rats.