中文摘要：

瞄准：为了在火腿的长手臂上定义普通删除的区域，染色体 7q 由杂合现象(LOH ) 和它的临床的 significance.METHODS 的损失用汉语连接了到主要的胃的癌：与 10cM 的平均标记密度在染色体 7q 上散布的九个微卫星标记被用来由 PCR 扩大为 LOH 检验 70 主要的胃的癌。PCR 产品被电气泳动在 polyacrylamide 胶化上分开。Genescan 和 Genotyper 软件被用来分析 LOH.RESULTS：有 7q 上的至少一个标记的 LOH 发生在 34.3%(12/50 ) 肿瘤。在他们之中，在 D7S486 和 D7S798 的 LOH 在 24.0% 是更高的(24/70 ) 并且 19.2%(5/26 ) 分别地。由统计分析，我们也在 7q (P【0.05 ) 上观察了明显的遗传型显型关联。没有淋巴，在在有淋巴节点转移的病人的 D7S486 的 LOH 的频率在那些比那显著地高节点转移(P=0.015 ).CONCLUSION：在有更差的预后的 D7S486 和它的关联的 LOH 的高发生建议可能有通常认为的肿瘤压制或在涉及胃的癌的肿瘤发生和前进的这个区域的基因。

英文摘要：

AIM： To define the common deleted region on the long arm of haman chromosome 7q linked to primary gastric carcinomas in Chinese by loss of heterozygosity （LOH） and its clinical significance. METHODS： Nine microsatellite markers distributed over chromosome 7q with an average marker density of 10cM were used to examine 70 primary gastric carcinomas for LOH by PCR amplification. The PCR products were separated by electrophoresis on polyacrylamide gel. Genescan and Genotyper softwares were used to analyze LOH. RESULTS： LOH with at least one marker on 7q occurred in 34.3% （12/50） of the tumors. Among them, LOH at D7S486 and D7S798 was higher in 24.0% （24/70） and 29.2% （5/26）, respectively. By statistical analysis we also observed an obvious genotype-phenotype correlation on 7q （P〈0.05）. The frequency of LOH at D7S486 in patients with lymph node metastasis was significantly higher than that in those without lymph node metastasis （P=0.015）. CONCLUSION： The high incidence of LOH at D7S486 and its correlation with poorer prognosis suggest that there might be putative tumor suppressor genes in this region involved in the tumorigenesis and progression of gastric carcinoma.