中文摘要：

目的：研究视网膜发育过程中血管内皮生长因子（VEGF）和色素上皮衍生因子（PEDF）所起的调节作用及其机制。方法：C57BL／6J小鼠从出生后7～12d饲养于75％氧环境，分别用抗VEGF、抗PEDF和抗CD31抗体作免疫组织化学标记视网膜切片。结果：持续处于高氧环境时，VEGF表达处于低水平而PEDF快速上升，血管生长减缓。高氧处理5d后回到普通环境，VEGF的表达迅速上升而PEDF却逐渐减少，出现了一过性的血管快速增长现象。结论：氧含量变化可调节VEGF和PEDF表达，参与视网膜血管发育。

英文摘要：

Objective：To determine the role of vascular endothelial growth factor （VEGF） and pigment epithelium derived factor （PEDF） in vascularization of the developing retina. Methods： Retinas were harvested at different postnatal days from C57BL/6J mice that have been maintained at ambient oxygen or exposed to 75% oxygen from P7 to P12. Immunostaining of VEGF, PEDF and CD31 was performed. Results：When the mice were exposed to 75% oxygen, the expression of VEGF was inhibited and expression of PEDF greatly enhanced; vascularization was slowed down. When exposed to the normal air condition 5 days later, the expression of VEGF increased sharply and peaked at P17. Conclusion： Oxygen can regulate the expression of VEGF and PEDF, and is involved in vascular development in retina.