采用Wolbachiawsp基因的1对通用引物81F和691R检测了神农架林区6种盖蛛的Wolbachia感染情况。检测结果表明,只有1头双线盖蛛(Neriene limbatinella)感染了Wolbachia,且对检测到的Wolbachia品系进行系统发育分析结果表明,双线盖蛛感染的Wolbachia品系属于Wolbachia品系分类的B组。
Woibachia infection was tested by wsp 81F and wsp 691R in 6 species of Nergene in Shennongjia. The result of PCR amplication indicated that only one individuals ofNeriene limbatineUa was infected by Wolbachia, and the phylogenetic analysis of wsp gene showed that Wolbachia strain detected in N. limbatineUa belonged to Wolbachia B supergroup.