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Infection of Wolbachia may improve the olfactory response of Drosophila
  • ISSN号:1001-6538
  • 期刊名称:科学通报(英文版)
  • 时间:0
  • 页码:1369-1375
  • 语言:中文
  • 分类:S435.112.3[农业科学—农业昆虫与害虫防治;农业科学—植物保护] S792.35[农业科学—林木遗传育种;农业科学—林学]
  • 作者机构:[1]College of Life Sciences, Hubei University, Wuhan 430062, China, [2]Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wu- han 430079, China
  • 相关基金:Supported by National Natural Science Foundation of China (Grant No. 30870284)
  • 相关项目:草间钻头蛛不同地理种群感染Wolbachia的分子检测及与杀虫剂敏感性的关系研究
中文摘要:

endosymbiotic 细菌 Wolbachia 感染各种各样的昆虫并且是首先为它操作主人复制的能力知道。最近的调查表明 Wolbachia 也影响体的房间的活动。我们这里由陷井方法和 T 迷宫证明 Wolbachia 感染有果蝇 simulans 的嗅觉的反应上的重要影响。感染 Wolbachia 的苍蝇花了更短的时间进入食物陷井并且比那些 uninfected 控制对在 T 迷宫的有气味的东西更敏感。嗅觉的反应的时间相对在苍蝇的 Wolbachia 密度。在被食物陷井在一更短的时间(不到 60 min ) 抓住的 15-day-old 苍蝇的 Wolbachia 密度比在很长时间(超过 100 min ) 拿的那些显著地高。量的 RT-PCR 证明在有快嗅觉的反应的苍蝇的重要有气味的东西受体基因 or83b 的抄本是显著地多于那些与慢嗅觉的反应。这些结果建议 Wolbachia 可能由在主机调整嗅相关的基因的表达式增加苍蝇的嗅觉的反应。

英文摘要:

The endosymbiotic bacterium Wolbachia infects various insects and is primarily known for its ability to manipulate host reproduction. Recent investigations reveal that Wolbachia also affects the activity of somatic cells. We here demonstrated by trap method and T-maze that Wolbachia infection had signifi- cant impact on the olfactory response of Drosophila simulans, Wolbachia-infected flies took shorter time to enter the food trap and were more sensitive to odorant in T-maze than those uninfected controls, The time of olfactory response was relative to Wolbachia density in flies, Wolbachia density in 15-day-old flies that were caught in a shorter time (less than 60 min) by food trap was significantly higher than those taken in a longer time (more than 100 min), Quantitative RT-PCR showed that the transcript of an important odorant receptor gene or83b in flies with fast olfactory response was sig- nificantly more than those with slow olfactory response, These results suggest that Wolbachia might increase olfactory response of flies by regulating the expression of olfaction-related genes in hosts.

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