中文摘要：

目的探讨香烟烟雾（Cigarette smoke extract，CSE）对清道夫受体A（scavenger receptor，SR-A）介导炎症相关性研究。方法制备CSE，5％、10％和15％浓度CSE刺激小鼠肺泡巨噬细胞（MH—S）6、12和24小时后，用实时荧光定量聚合酶链反应（PCR）技术检测SR-A表达水平，酶联免疫吸附测定（ELISA）法测定炎症因子IL-6、肿瘤坏死因子α（TNF-α）表达水平。结果CSE刺激巨噬细胞6小时、12小时和24小时后，与空白对照组相比，SR—A表达水平升高，SR—A表达水平随着研究的时间延长而逐渐升高（P〈0．01）。而相对应时间IL-6和TNF-α表达水平分别与空白对照相比，都显著升高且差异有统计学意义（P〈0．01）。通过直线相关分析统计得出SR—A表达水平与IL-6表达水平呈正相关（P〈0．05），与TNF-α表达水平亦呈正相关（P〈0．01）。结论香烟烟雾刺激肺泡巨噬细胞后SR—A表达水平升高，且SR-A表达与炎症因子IL-6、TNF-α表达成正相关，SR—A可能参与介导CSE诱导的肺泡巨噬细胞的炎症反应。

英文摘要：

Objective Correlation between inflammation mediated by cigarette smoke extract （CSE） on the scavenger receptor was detected. Methods CSE was prepared and mouse alveolar macrophage （MH-S） was stimulated by different concentration of CSE （ 5%, 10 %, 15%） and different time （6,12,24 hours）, Real-time polymerase chain reaction detected the SR-A expression levels, enzyme- linked immunosorbent assay （ELISA） measured the level of IL-6 and TNF-α. Results CSE stimulated MH-S for 6 hours,12 hours and 24 hours,the expression level of SR-A was higher than that in control group and increased steadily with extending time （P 〈 0.01 ）. IL-6 and TNF-α expression levels, compared with the control, were significantly increased （ P 〈 0.01）. The expression level of SR-A was positively correlated with concentration of IL-6 and TNF-α by linear correlation analysis （ P 〈 0.05）. Conclusions There is an up-regulated expression of SR-A by CSE-stimulated mouse alveolar macrophage, and SR-A expression level is positively correlated with levels of IL-6 and TNF-α. SR-A might involved in mediating the alveolar maerophage inflammatory response induced by CSE.