中文摘要：

【目的】研究粘质沙雷氏菌（Serratia marcecens JNB5-1）在不同发酵温度（28℃和370C）条件下蛋白质组的差异表达，探究粘质沙雷氏菌合成灵菌红素受温度调控的原因。【方法】利用二维凝胶电泳（2-DE）和基质辅助激光解析电离飞行时间串联质谱技术（MALDI-TOF-MS）分离和鉴定粘质沙雷氏菌在不同发酵温度（28℃和37℃）条件下的差异蛋白；再利用实时荧光定量PCR（RT-qPCR）进一步分析挑选出的七个差异蛋白编码基因的转录水平。【结果】2-DE图谱显示，共鉴定到16个显著差异的蛋白点。其中，灵菌红素合成途径中的氧甲基转移酶（PigF）、氧化还原酶（PigN）和参与灵菌红素前体物质（脯氨酸、甲硫氨酸、丝氨酸、二辛烯醛和丙二酰．CoA等）代谢的相关蛋白（酶）的表达量在37℃条件下都出现极显著的下降；热休克蛋白（Hsp60）、超氧化物歧化酶（Superoxide dismutase）等应急性蛋白在37℃条件下表达上调。通过RT-qPCR证实，氧甲基转移酶、氧化还原酶和转酮醇酶基因（transketolase）在37％条件下的转录水平均低于28℃条件下的。【结论】S．marcecens JNB5-1在相对低温（28℃）条件下产灵菌红素，相对高温（37℃）条件下不产灵菌红素。可能是高温（37℃）显著抑制了灵菌红素合成相关酶的表达。

英文摘要：

[ Objective ] To study the effect of temperature on prodigiosin synthesis in Serratia marcecens JNBS-1. [Methods] Different proteins obtained after culturing S. marcecens JNBS-lat 28℃ and 37℃ were separated and identified through two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. Subsequently, 7 of the proteins were further analyzed by real-time qPCR. [ Results] We identified 16 different proteins. Among them, prodigiosin biosynthetic enzymes （ O-methyl transferase and Oxidoreductase） and proteins related with the precursor substances （proine, methionine, serine, 2-Octenal and Malonyl-CoA） of the prodigiosin biosynthetic were significantly down-expressed at 37 ℃. Heat shock protein （Hsp60） and superoxide dismutase were upexpressed at 37℃. The results of real-time qPCR show that the mRNA transcriptional levels of o-methyl transferase, oxidoreductase and transketolase at 37℃ were all lower than that at 28 ℃. [ Conclusion] S. marcecens JNB5-1 could produce prodigiosin at lower temperature （28 ℃） but not at higher temperature （37℃）. Higher temperature probably inhibited the expression of enzymes related with prodigiosin biosynthesis.