目的:探讨肥胖相关新基因NYGGF4在人脂肪组织中转录本的大小及是否存在勇切异构体。方法:采用Northern blot技术检测NYGGF4基凶在人脂肪组织中的表达情况。结果:检测到一条大小1.5kb左右的条带,与数据库中NYGGF4 mRNA长度一致,未检测到其他转录本。结论:NYGGF4基凶在人脂肪组织中的转录本大小约为1.5kb,并不存在剪切异构体。
Objective: To explore the size of transcripts of NYGGF4 gene and other transcript variants being or not. Methods: The expression of NYGGF4 gene in human adipose tissues was detected by Northern blot. Results: A 1.5 kb band was detected, and no other transcript was found. Conclusion: NYGGF4 gene displays only one transcript whose size is 1.5 kb in human adipose tissues without any other transcript variants.