中文摘要：

目的：观察leptin对人成熟脂肪细胞中NYGGF4基因表达的调节作用。方法：体外培养人前体脂肪细胞，在诱导人前体脂肪细胞分化成熟的基础上，应用100ng／ml人重组leptin分别干预成熟脂肪细胞6、24h，采用实时荧光定量RT—PCR技术检测成熟脂肪细胞中NYGGF4基因mRNA的表达水平。结果：100ng／ml leptin干预人成熟脂肪细胞6h后．NYGGF4基因mRNA的相对表达量为0．000476±0．000178，显著低于对照组0．00114±0．000275，P〈0．05；干预24hNYGGF4基因mRNA的相对表达量为0．000835±0．000211，也显著低于对照组0．001419±0．000193，P〈0．05。结论：leptin能显著下调成熟脂肪细胞中NYGGF4基因的表达。

英文摘要：

Objective：To explore the regulative role of leptin on NYGGF4 gene expression in matured human adipocytes. Methods： Human preadipocytes were cultured in vitro and differentiated into matured adipocytes. The matured adipocytes were treated with 100 ng/ml leptin for 6 hours and 24 hours, respectively. Total RNA from these adipocytes was extracted and the levels of N3fGGF4 mRNA expression were evaluated by real-time quantitative RT-PCR. Results：Treatment with 100 ng/ml leptin for 6 hours or 24 hours significantly decreased NYGGF4 gene mRNA expression in human matured adipocytes （P 〈 0.05）. Condusion：Leptin could downregulate the expression of NYGGF4 gene mRNA in human matured adipocytes.