中文摘要：

目的探索更年期高血压燥证大鼠模型的建立方法。方法 2014年1—3月选取雌性自发性高血压大鼠（SHR）12只,采用摸球法将SHR随机分为更年期高血压组和更年期高血压燥证造模组,每组6只。采用外科手术法摘除两组大鼠双侧卵巢,制备更年期高血压大鼠。对更年期高血压燥证造模组大鼠施加人工燥证环境,饲喂西北燥证食物进行造模。观察大鼠生物学特征（毛色、精神状态、兴奋度、进食量、饮水量、体质量、大小便性状、舌象、爪象等）、血清神经递质〔促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素（ACTH）、去甲肾上腺素（NE）、多巴胺（DA）、5-羟色胺（5-HT）〕及激素〔雌二醇（E2）、睾酮（T）〕水平和脏器（肝脏、脾脏、肾上腺、下丘脑）组织结构。结果更年期高血压燥证造模组大鼠较更年期高血压组体质量降低,饮水量增加,尿量减少（P〈0.05）。更年期高血压燥证造模组大鼠血清CRH、NE、DA、5-HT水平较更年期高血压组升高（P〈0.05）。两组大鼠ACTH水平比较,差异无统计学意义（P〉0.05）。更年期高血压燥证造模组大鼠较更年期高血压组血清E2、T水平降低,E2/T升高（P〈0.05）。两组大鼠肝脏、脾脏、肾上腺、下丘脑组织结构均发生明显改变。结论通过对更年期高血压大鼠施加人工燥证环境,给予西北燥证食物,控制饮水量及进食量,可成功建立更年期高血压燥证大鼠模型。

英文摘要：

Objective To explore the method of the establishment of climacteric hypertension dryness syndrome model in rats. Methods A total of 12 female spontaneously hypertensive rats（ SHR） were selected as study subjects from January to March in 2014,SHR were randomly divided into climacteric hypertension group and climacteric hypertension dryness syndrome model group,there were 6 rats in each group. The bilateral ovaries of rats were removed by surgery,thus climacteric hypertension rats were obtained. Rats in climacteric hypertension dryness syndrome model group were placed in artificial dryness syndrome environment,and were fed with food from northwest area which may cause dryness syndrome. The biological characteristics（ coat color,mental state, degrees of excitement, food intake, water intake, body mass, characters of urine and stool, tongue picture,claws picture）,serum levels of neurotransmitters 〔corticotropin- releasing hormone（ CRH）, adrenocorticotrophic hormone（ ACTH）, norepinephrine（ NE）, dopamine（ DA）,5- hydroxytryptamine（ 5- HT） 〕 and hormones 〔estradiol（ E2）,testosterone（ T） 〕,and histological changes of organs（ liver,spleen,adrenal gland,and hypothalamus） were observed and recorded. Results Compared with rats in climacteric hypertension group,the body mass and urine volume were significantly decreased, and water intake was significantly increased among rats in climacteric hypertension dryness syndrome model group, the differences were statistically significant（ P〈0. 05）. Serum levels of CRH, NE, DA and 5- HT in climacteric hypertension dryness syndrome model group were significantly higher than those in climacteric hypertension group（ P〈0. 05）. There was no significant difference in ACTH level between two groups（ P〉0. 05）. Compared with rats in climacteric hypertension group,serum levels of E2 and T were significantly decreased,and E2/ T was significantly increased among rats in climacteric hypertension dryness syndrome model group,the differe