中文摘要：

AIM： To analyze the hepatitis B virus （HBV） charactersin China, as well as the correlation between severalHBV mutation and hepatitis symptoms.METHODS： A total of 1148 HBV genome sequencesfrom patients throughout China were collected via theNational Center For Biotechnology Information database（information including： genotype, territory and clinicalstatus）. HBV genotypes were classified by a directreference from the Genbank sequence annotation,phylogenetic tree and online software analysis （http：//www.ncbi.nlm.nih.gov/projects/genotyping/formpage.cgi）. The phylogenetic tree was constructed based onthe neighbor-joining method by MEGA5.0 software.HBV sequences were grouped based on phylogenetictree and the distance between the groups wascalculated by using the computer between group meandistance methods. Seven hundred and twelve HBVsequences with clear annotation of clinical symptomswere selected to analyses the correlation of mutationand clinical symptoms. Characteristics of sequences were analyzed by using DNAStar and BioEdit softwarepackages. The codon usage bias and RNA secondarystructures analysis were performed by RNAdrawsoftware. Recombination analysis was performed byusing Simplot software.RESULTS： In China, HBV genotype C was thepredominant in Northeastern, genotype B waspredominant in Central Southern areas, genotype Band C were both dominant in Southwestern areas,and the recombinant genotype C/D was predominantin Northwestern areas. C2 and B2 were identified asthe two major sub-genotypes, FJ386674 might be aputative sub-genotype as B10. The basal core promoterdouble mutation and pre-C mutation showed varioussignificant differences between hepatitis symptoms. Inaddition to ATG, many other HBV initiation codons alsoexist. HBV has codon usage bias; the termination codonof X, C and P open reading frames （ORF） were TAA,TAG, and TGA, respectively. The major stop codons ofS-ORF were TAA （96.45%） and TGA （83.60%） in B2and C2 subtype, respectively.CONCLUSION： This study recapitu