研究不同质量浓度的1,4-二氯苯(1,4-DCB)对蛋白核小球藻(Chlorella pyrenoidosa)的生长状况、蛋白质含量、叶绿素含量、超氧化物歧化酶活性(SOD酶活性)和脂质过氧化丙二醛(MDA)含量的影响,检测1,4-DCB对海洋微藻的毒性效应及其毒性机理.结果表明:1,4-DCB对蛋白核小球藻的生长有一定的抑制作用,该效应表现出一定的浓度和时间依赖性;1,4-DCB处理4d后,蛋白核小球藻细胞蛋白质含量和叶绿素含量下降,SOD酶活性降低,MDA含量升高,这表明1,4-DCB通过产生活性氧自由基引起脂质及其他生物大分子的氧化损伤可能是1,4-DCB对蛋白核小球藻产生毒害效应的主要机制,受有机物污染后藻类作为饵料的价值降低。
Toxic effects of 1,4-dichlorobenzene (1,4-DCB) on Chlorella pyrenoidosa were studied through the changes of growth, the protein contents, and the chlorophylla contents of algae. And the toxic mechanisms of Chlorella pyrenoidosa were analyzed by the activities of SOD enzymes and the MDA contents of algae. The results showed that the growth of algae was inhibited by the 1,4-DCB in treated time-response and dose-response manners. The protein contents, the chlorophylla contents, especially the contents of the chlorophylla a, were decreased in Chlorella pyrenoidosa cells treated by 1,4-DCB for 4 d. The activities of SOD were decreased and the contents of MDA increased in doseresponse manners. The resuhs suggested that primary mechanism of 1,4-DCB on Chlorella tryrenoidosa cells may be related to the oxidation damage of lipid and other biological large molecules caused by 1,4-DCB. The pollution of 1,4-DCB decreased the values of Chlorella pyrenoidosa as the food of organisms.