中文摘要：

组蛋白去乙酰化酶（histone deacetylase,HDAC）通过调节组蛋白乙酰化修饰参与调控基因表达.研究发现多种HDAC参与成脂分化,但其机制尚不清楚.本研究探讨间充质干细胞C3H10T1/2成脂分化过程中HDAC的表达变化及其对成脂分化的影响.本研究首先建立了C3H10T1/2体外成脂分化的模型,并以油红O染色鉴定成功诱导成脂分化.PCR检测C3H10T1/2细胞成脂分化过程中11种HDAC的变化趋势,发现成脂分化过程中,HDAC1、2、5、9和10的mRNA表达量下降,而HDAC3、6、8和11的mRNA表达量明显上升,其中HDAC11上升最为显著.进一步通过RNA干扰沉默HDAC11表达,PCR检测成脂分化的关键转录因子PPARγ2和成脂标志物Perilipin、Adipoq的mRNA表达量下降,但Fabp4表达变化不明显.油红O染色结果表明,诱导C3H10T1/2成脂分化过程中,干扰HDAC11表达,胞浆内脂滴形成数量减少,成脂分化受到抑制.实验结果提示,C3H10T1/2细胞成脂分化伴随着多种HDAC表达的变化,其中HDAC11的增加最显著,干扰HDAC11的表达可以抑制C3H10T1/2细胞的成脂分化.

英文摘要：

Histone deacetylase （HDAC） regulates gene expression by controlling histone acetylation. It has been reported that HDACs participated in adipogenic differentiation, but the underlying mechanisms were less understood. To study the effects of HDACs on adipogenic differentiation, oil red O staining was used for the assay of adipogenesls of mesenchymal stem cells C3H10TI/2 in vitro. RT-PCR was used to detect the changes in the expression of HDACll during adipogenic differentiation, and the results showed large differences in the expression levels of each kind. HDAC3, 6, 8 and 11 were increased significantly （with HDACll as the most）, while HDAC1, 2, 5, 9 and 10 were decreased. No obvious change was observed for Fabp4. Transfection of siRNA-HDAC11 suppressedadipogenetic differentiation with down-regulated level of the key adipogenic transcription factor of PPARγ2, and adipogenic markers of perilipin and adipoq. In conclusion, the expression of HDACs varied during the adipogenic differentiation of C3H10T1/2, and increased expression of HDACll might facilitate adipogenesis.