中文摘要：

目的 ：观察羟甲基戊二酰辅酶A还原酶降解蛋白1（HMG-Co A reductase degradation protein 1,HRD1）在人脐静脉血管内皮细胞（human umbilical vein endothelial cells,HUVEC）内的表达情况,探讨HRD1对氧化型低密度脂蛋白（oxidized low density lipoprotein,ox LDL）引起的血管内皮细胞凋亡及细胞内脂质沉积的影响。方法 ：免疫荧光检测HRD1在动脉粥样硬化患者血管组织中的表达。同时体外传代培养HUVEC,ox LDL刺激细胞后Western blot法观察HRD1的表达情况。Ad-HRD1感染ox LDL刺激后的HUVEC,Western blot法观察HRD1对细胞凋亡的影响,油红O染色法观察HRD1对细胞内脂滴生成的影响。结果：HRD1表达于动脉粥样硬化患者血管组织中;也表达于ox LDL刺激后的HUVEC中;Ad-HRD1感染ox LDL刺激后的HUVEC,Cleaved PARP和Cleaved Caspase 3表达降低并且细胞内脂滴形成减少。结论：HRD1可抑制ox LDL导致的内皮细胞凋亡和细胞内脂质沉积。

英文摘要：

Objective：To explore the role of HRD1 in apoptosis and lipid deposition caused by ox LDL in human umbilical vein endothelial cells（HUVECs）. Methods：HUVECs were incubated with 0 μg / ml ox LDL as control group,or with 50 μg / ml ox LDL as ox LDL group. Western blot was used to analyze expression of HRD1 protein. HRD1 expression in vascular tissue was detected by immunofluorescence analysis. HRD1 effects on cell apoptosis and lipids deposition of HUVEC was detected by western blot and oil red o staining analysis. Results：Expression of HRD1 protein decreased significantly in ox LDL group than that in control group.Meanwhile,HRD1 also expressed in the athrosclerostic plaques,and predominantly in the endothelial cells. ox LDL induced the significant increases in apoptosis and lipids deposition of HUVEC. While the HRD1 overexpression by the infection of Ad-HRD1 markedly inhibited the Cleaved PARP and Cleaved Caspase 3 expression and lipids deposition caused by ox LDL. Conclusion：HRD1inhibited the apoptosis and lipids deposition of HUVEC induced by ox LDL.