中文摘要：

目的：了解系统性红斑狼疮（SLE）患者外周血单核细胞主要组织相容性复合体（MHC）Ⅰ类链相关分子（MⅠCs）、IL-15表达水平变化及其潜在机制。方法：收集20例SLE患者及20例健康志愿者外周血,分离单核细胞及血清,采用流式细胞术检测单核细胞表面MⅠCs及mIL-15的表达,EILIS法检测血清中干扰素-γ（INF-γ）、干扰素-α（INF-α）浓度,并对单核细胞表面MⅠCs表达水平与血清INF-γ浓度作相关性分析。进一步以SLE患者血清与健康志愿者单核细胞体外孵育,在有或无抗INF-γR存在的情况下,检测单核细胞表面MⅠCs分子的表达情况。结果：SLE患者血清IFN-γ浓度与健康对照组相比有显著性差异,其中IFN-γ在SLE患者为（429.7±145.0）pg/ml[健康对照为（73.8±18.9）pg/ml],二者有显著性差异（P〈0.01）;单核细胞MⅠCs及mIL-15的阳性率分别为（37.0±18.1）%和（36.0±7.3）%,而健康志愿者单核细胞MⅠCs及mIL-15的阳性率为（7.2±2.4）%和（7.6±2.5）%。SLE患者MⅠCs及mIL-15阳性率显著高于健康志愿者（P〈0.01）;SLE患者单核细胞MⅠCs分子表达水平与血清INF-γ浓度呈正相关;SLE患者血清中高浓度的IFN-γ可以刺激正常人单核细胞表达高水平的MⅠCs及mIL-15。结论：SLE患者血清INF-γ浓度异常升高,导致外周血单核细胞表面MⅠCs及IL-15异常高表达。

英文摘要：

Objective： To investigate the surface expression level of MHC class I chain-relate molecules （MICs） and membrane IL-15 on peripheral blood monocytes in patients with system lupus ery- thematosus （SLE）. Methods： Twenty patients with SLE and 20 healthy volunteers were included in this study. Peripheral blood monouclear cells （PBMCs） were harvested by density gradient centrifugation, and M ICs and IL-15 on monocytes were detected by flow cytometry. Serum con- centration of IFN-γ were examined by ELISA. The relationship between monocyte MICs and se- rum IFN-γ were analyzed. Purified monocytes from healthy donors were incubated with sera from SLE patients in the presence or absence of blocked anti-INF-TR antibodies, and the MICs expression on monocytes were further studied. Results： The concentration of IFN-γ in SLE patients （150.3-669.5 pg/ml, [-429. 7±145. 01 pg/ml） was significantly higher than in normal controls （18.8-88.2 pg/ml, [-73.8±18.91 pg/ml）. The expression of MICs on monocytes was （7.2±2.4）% in normal controls versus （37.0±18.1）% in SLE patients, mIL-15 expression of CD14＋ monoeytes in SLE patients was （36.0±7.3）%, which is significantly higher than those in normal controls （[7. 6± 2. 5]%）. The concentration of IFN-γ in patients was significantly correlated with the expression of M[Cs on monocytes. Sera from SLE patients can promote the expression of MICs and mIL-15 on monocytes, but this effects can be prevented in the condition of IFN-γ blocking. Conclusion： Increased sera IFN-γ in patients with SLE is responsible for the aberrant expression of MICs and mIL-15 on monocytes.