中文摘要：

目的：观察阿魏酸对β-淀粉样蛋白25-35（βamyloid peptide25-35,Aβ25-35）诱导的阿尔兹海默病（Alzheimer disease,AD）模型细胞的抗氧化能力的影响,探讨当归防治AD的作用及机制。方法：用Aβ25-35诱导PC12细胞凋亡,以不同浓度阿魏酸对抗干预,以MTT比色法观测阿魏酸对AD模型细胞增殖的保护作用,以比色法检测阿魏酸对AD模型细胞培养液中超氧化物歧化酶（SOD）、丙二醛（MDA）、乳酸脱氢酶（LDH）的影响。用RT-PCR法检测Apbb1,乙酰胆碱酯酶（Ache）基因的表达。结果：阿魏酸对损伤的PC12细胞的存活率有提高作用,阿魏酸在2 mmol/L时可明显降低模型细胞培养液中MDA、LDH含量,提高SOD活力,差异有统计学意义（P〈0.01）。阿魏酸可下调Apbb1和Ache的mRNA表达,差异有统计学意义（P〈0.01）。结论：阿魏酸对Aβ25-35损伤的PC12细胞有保护作用,机制可能与提高细胞抗氧化能力相关,与下调Apbb1、Ache的mRNA表达量相关。

英文摘要：

Objective： To observe the effect of Ferulic acid to the antioxidant capacity of model cell induced by Aβ25-35. To explore the effect and mechanism of angelica prevention and treatment of AD. Methods： We separately induced PC12 cells by using Aβ25-35 to against and intervened Ferulic acid with different concentration. MTT colorimetric method was used to detect and observation protection effect of Ferulic acid to cell proliferation on two kinds of AD models. Colorimetric method was used to detect the influence of Ferulic acid to activity of lactic dehydrogenase,MDA content,SOD activity and LDH content in damaged PC12 cell culture. We explored the gene expression of Apbb1 and Ache by RT-pcr method. Results： Ferulic acid increased the survival rate of damaged PC12 cells and the peak was at 2 mmol / L in Aβ25-35 models which can obviously decrease MDA content and improve SOD activity（ P〈0. 01）. Ferulic acid can cut mRNA expression of Ache and Apbb1（ P〈0. 01）. Conclusion： Ferulic acid can prevent PC12 cell from ability of the cell and Ache and mRNA expression of Ache and Apbb1.