中文摘要：

t圈套蛋白质 SNAP-25 ( 25 kDa 的 联系synaptosome 的蛋白质)在在 exocytosis.To 期间调整在泡和血浆膜之间的熔化起一个必要作用克隆并且描绘 SNAP-25 基因，在在海洋的硬骨鱼类的圈套蛋白质的功能的学习的第一步，是由日本海栖息的 RT-PCR 和 RACE-PCRamplification 获得海栖息 SNAP-25 ( SPsn25 )的 cDNA 。831bp 的全身的 cDNA 包含 219bp 的 615 bp， coding204 氨酸残余，和 5'UTR 的 CDS。生物信息的分析与 SNAP-25a isoform 和份额揭示了那 SPsn25corresponds 有一条金鱼和 azebrafish 的 SNAP-25a 的 91.1% 身份。在 mRNA 的 SPsn25 表示和在日本海栖息的蛋白质层次通过半量的 RT-PCR 和西方的污点试金被识别了。一起，这些数据再证实了鱼 SNAP-25 基因表达式的神经织物特性。

英文摘要：

The t-SNARE protein SNAP-25 （synaptosome-associated protein of 25 kDa） plays an essential role in regulating fusion between the vesicle and plasma membranes during exocytosis. To clone and characterize SNAP-25 gene, the first step in the functional study of SNARE proteins in marine teleostean, was to obtain the cDNA of sea perch SNAP-25 （SPsn25） by RT-PCR and RACE-PCR amplification of a Japanese sea perch. The full-length cDNA of 831 bp contains a CDS of 615 bp, coding 204 amino acid residues, and a 5＇UTR of 219bp. Bioinformatic analysis revealed that SPsn25 corresponds with SNAP-25a isoform and shares 91.1% identity with SNAP-25a of a goldfish and a zebrafish. The SPsn25 expression in both mRNA and protein levels in the Japanese sea perch had been identified through semi-quantitative RT-PCR and Western Blot assay. Together, these data again confirmed the nerve tissue specificity of the fish SNAP-25 gene expression.