以便在鱼向离子隧道的恰当的证据提供有免疫力的反应,整个房间补丁夹钳技术在比目鱼之类(Scophthalmus 敲奏编钟的一种技巧) 为钾离子隧道学习被采用。淋巴细胞被 Percoll 密度坡度 centrifugation 从外部血样品孤立,并且隧道的 electrophysiological 人物被分析。在记录房间,激活隧道的电压 ? 42.5 潩獮椠 ? 桴 ? 楨桧洠牡桳愠摮琠敨洠湩浩浵瘠污敵 ? 湩漠数 ? 慢敲映慬? ü
In order to provide pertinent evidence of ion channel with immune response in the fish, whole cell patch-clamp technique was employed for potassium ion channel study in turbot (Scophthalmus maximus). Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples, and electrophysiological characters of the channel were analyzed. In the recorded cells, activated voltage of the channels was -42.5±3.7 mV and the average peak current was 313.12±28.2 pA. The channel was identified as voltage dependent, the current was outward and it could be inhibited by 10 mmol/dma TEA or 5 mmol/dm^3 4-AP, a specific potassium channel inhibitor, identifying the existence of potassium channel in peripheral lymphocytes of the turbot.