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Potassium channel in peripheral blood lymphocytes of turbot ( Scophthalmus maximus)
  • ISSN号:0253-505X
  • 期刊名称:《海洋学报:英文版》
  • 时间:0
  • 分类:Q73[生物学—分子生物学] TS48[农业科学—烟草工业;轻工技术与工程]
  • 作者机构:[1]College of Marine Life Sciences, Shandong University at Weihai, Weihai 264209, China, [2]Marine Bioactive Substances Laboratory, the First Institute of Oceanography, StateOceanic Administration, Qingdao 266061, China
  • 相关基金:The National Natural Science Foundation of China under contract Nos 40476060 and 40876089; the State Key Basic Research Program under contract No. 2006CB101805.
中文摘要:

以便在鱼向离子隧道的恰当的证据提供有免疫力的反应,整个房间补丁夹钳技术在比目鱼之类(Scophthalmus 敲奏编钟的一种技巧) 为钾离子隧道学习被采用。淋巴细胞被 Percoll 密度坡度 centrifugation 从外部血样品孤立,并且隧道的 electrophysiological 人物被分析。在记录房间,激活隧道的电压 ? 42.5 潩獮椠 ? 桴 ? 楨桧洠牡桳愠摮琠敨洠湩浩浵瘠污敵 ? 湩漠数 ? 慢敲映慬? ü

英文摘要:

In order to provide pertinent evidence of ion channel with immune response in the fish, whole cell patch-clamp technique was employed for potassium ion channel study in turbot (Scophthalmus maximus). Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples, and electrophysiological characters of the channel were analyzed. In the recorded cells, activated voltage of the channels was -42.5±3.7 mV and the average peak current was 313.12±28.2 pA. The channel was identified as voltage dependent, the current was outward and it could be inhibited by 10 mmol/dma TEA or 5 mmol/dm^3 4-AP, a specific potassium channel inhibitor, identifying the existence of potassium channel in peripheral lymphocytes of the turbot.

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期刊信息
  • 《海洋学报:英文版》
  • 主管单位:
  • 主办单位:中国海洋学会
  • 主编:潘德炉
  • 地址:北京海淀大慧寺路8号
  • 邮编:100081
  • 邮箱:hyxbe@263.net
  • 电话:010-62179976
  • 国际标准刊号:ISSN:0253-505X
  • 国内统一刊号:ISSN:11-2056/P
  • 邮发代号:82-285
  • 获奖情况:
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),荷兰文摘与引文数据库,美国剑桥科学文摘,美国科学引文索引(扩展库),英国科学文摘数据库,英国动物学记录
  • 被引量:331