中文摘要：

目的：建立天胡荽属植物的积雪草苷和羟基积雪草苷HPLC含量分析的方法,为开发利用天胡荽属植物药用资源奠定基础。方法：以积雪草苷和羟基积雪草苷为指标成分,采用高效液相色谱法,以Symmetry C18（4.6 mm×250 mm,5μm）为色谱柱;乙腈-水（29∶71,v/v）为流动相;流速0.8 mL/min;柱温30℃;检测波长210 nm,外标法进行测定。结果：积雪草苷的回归方程为：Y=34 958X＋154 822 R2=0.999 1（n=8）,羟基积雪草苷的回归方程为：Y=114 318X-21 002 R2=0.999 2（n=8）,结果表明线性关系与分离度良好,平均加样回收率为99.98%、100.53%,RSD为1.86%、2.31%。结论：该方法稳定性、重复性良好,可做为天胡荽属药用资源的评价方法之一,实验结果为野生与栽培资源的开发与利用奠定了基础。

英文摘要：

Objective： To establish a stable method for the content determination of asiaticoside and madecassoside in Hydrocotyle.And lay a foundation for the further study.Method： The HPLC was performed using Symmetry C18 column（4.6 mm×250 mm,5 μm） and acetonitrile-water（29∶71） as the mobile phase.The flow rate was 0.8mL/min,the detection wavelength was 210 nm,and the column temperature was 30 ℃.The external standard method was used as quantitative analysis.Results： the regression equation of asiaticoside was Y=34 958X＋154 822 R2=0.999 1（n=8）,the regression equation of madecassoside was Y=114 318X-21 002 R2=0.999 2（n=8）;the average recovery was 99.98%、100.53%,RSD was 1.86%、2.31%.Conclusion： The method is stable and reliable.It can be used for quality control of Hydrocotyle.And the result provides scientific basis for exploiting wild and plant resources.