中文摘要：

【目的】观察中药益气血补肝肾方对胚胎着床障碍小鼠子宫内膜整合素β3表达的影响,探讨其改善胚胎着床的分子基础。【方法】将90只雌鼠随机分为正常组、模型组和治疗组3组,每组30只,其中,治疗组小鼠予以中药益气血补肝肾方治疗（包括经后增殖方8 mg/kg和促黄体方8 mg/kg）,在妊娠第4天（Pd4）上午8∶00采用米非司酮诱导模型组和治疗组小鼠胚胎着床障碍,12 h后处死小鼠（每组20只）,剖腹取小鼠子宫,小心刮取子宫内膜,采用Real-time PCR和Western Blot的方法分别在m RNA和蛋白水平上检测整合素β3在各组小鼠子宫内膜中的表达情况。其余小鼠（每组10只）于Pd4剖腹取小鼠子宫,固定于体积分数2.5%戊二醛溶液中,行扫描电镜观察。【结果】Real-time RT-PCR和Western Blot结果显示：模型组整合素β3 m RNA与蛋白表达显著低于正常组（P〈0.05）;治疗组可显著升高整合素β3 m RNA与蛋白表达水平,与模型组比较,差异均有统计学意义（P〈0.05）。胞饮突在模型组中的表达少于治疗组和正常组,而胞饮突在治疗组和正常组的表达相似。【结论】中药益气血补肝肾方可上调子宫内膜中整合素β3的表达,对胞饮突在Pd4小鼠子宫内膜中的表达有正调节作用。

英文摘要：

Objective To observe the effect of Yiqixue Buganshen Recipes（YBR）,the compound recipes of Chinese herbal medicine with the actions of replenishing Qi and blood and tonifying liver and kidney, on the endometrial integrin β3 of the mice with embryo implantation dysfunction, and to explore the molecular mechanism of YBR in improving embryo implantation. Methods Ninety mice were divided normal group,model group, and YBR group, 30 mice in each group. YBR group was given Jinghou Zengzhi granules 8mg/kg for promoting post-menstruation proliferation and Cuhuangti granules 8mg/kg for promoting the formation of corpora luteum. At 8∶00 am of pregnant day 4（Pd4）,subcutaneous injection of mifepristone was used for inducing embryo implantation dysfunction in the model group and YBR group. Twenty mice from each group were executed12 hours after subcutaneous injection of mifepristone, and the uterus was isolated for the detection of mice endometrial integrin β3 m RNA and protein expression with real-time RT-PCR and Western blotting method respectively. Ten mice from each group at Pd4 were executed, and the uterus was isolated and then fixed in2.5% glutaraldehyde solution for the detection of the formation of pinopode in mice endometrium under scanning electron microscope. Results The detection by real-time PCR and Western blotting method revealed that integrinβ3 m RNA and protein expression levels of the model group were significantly lower than those of the normal group（P〈0.05）,and YBR group had higher integrin β3 m RNA and protein expression levels than the model group（P〈0.05）. The formation of pinopodes in the model group was less than the normal group and YBR group,but the number of formed pinopodes in YBR group was similar to that in the normal group. Conclusion YBR can up-regulate the expression of integrin β3 in the endometrium of mice with embryo implantation dysfunction, and have positive regulatory effect on the formation of pinopodes on Pd4.