中文摘要：

目的探讨继发不孕患者行部分卵子单精子卵泡浆内显微注射（half intracytoplasmic sperm injection，half—ICSI）的适应证。方法回顾性分析继发不孕患者行部分卵子单精子卵泡浆内显微注射共139个周期，按体外授精（IVF）受精率≤50％和〉50％分组，比较两组患者年龄、不孕年限、受精率、临床妊娠率等情况；分析IVF受精失败12周期的相关情况。结果IVF受精率≤50％组的女方年龄较大，男方因素比例高，ICSI受精率低，获卵数及冷冻胚胎均少于受精率〉50％组（P〈0．05）。IVF受精失败的12周期与IVF受精率〉50％组比较也得出相似结果。结论对于继发不孕患者，女方年龄大，男方精液为临界值，若为原发不育者，行half—ICSI可能帮助其减少受精失败的概率。

英文摘要：

Objective To investigate the indications of half intracytoplasmic sperm injection （half - ICSI） for secondary infertile patients. Methods A retrospective study of 139 cycles from 2005 to May 2011 was performed. These cycles were divided into two groups according to the 1VF fertilization rate. The age, ICSI fertilization rate, pregnancy rate, and etc. were compared. Results In Group A （IVF fertilization rate≤50% ）, the age and male factor ratio were signifi- cantly higher than those in Group B （ IVF fertilization rate 〉 50% ）, with significantly lower ICSI fertilization rate and retrieved eggs （ P 〈 0. 05 ）. Conclusion Half - ICSI provides significant rescued effects on fertilization rate for the secondary infertile cases with old female age, borderline semen quality and especially primary infertility.