中文摘要：

目的：鉴定FHL2与Id家族蛋白成员之间的相互作用及分子表位。方法：GST-pulldown方法检测。结果：FHL2与Id家族的4个成员蛋白均存在直接的相互作用关系，FHL2蛋白中的第二个LIM结构域在FHL2／Id相互作用中是必需的，Id蛋白N端结构区域在介导FHL2／Id相互作用中是必需的。结论：FHL2是一个新识别的Id蛋白广谱相互作用因子，FHL2可能参与Id介导的多种生物学效应以及肿瘤发生与进展。

英文摘要：

Objective： To investigate the interaction between FHL2 and Id proteins and its corresponding epitope. Methods： Interaction between FHL2 and Id proteins and the corresponding epitope conferring this association were detected by GST pulldown assays. Results： FHL2 directly interacted with all Id proteins. The second LIM domain containing FHI2 or N ter- minal region in Id proteins was essential for the direct binding of FHL2 to ld proteins. Conclusion： FHL2 is a novel and broad interacting factor of the ld proteins. Through cooperating with Id proteins, FHL2 may be involved in multiple biological effects and tumorigenesis mediated by Id proteins.