中文摘要：

【目的】观察平原短期高强度间歇运动训练（high-intensity interval training,HIT）对青年男性急进高原淋巴细胞线粒体DNA氧化损伤的影响。【方法】30例世居平原青年男性随机分为无运动训练组（NT,n=15）和高强度间歇运动训练组（HIT,n=15）。HIT组受试者进行HIT训练,以90%~95%心率贮备强度快跑3 min,间歇以45%~50%心率贮备强度慢跑3 min,重复6个循环,7次/周,共2周。训练后所有受试者同批次急进高原,在移居高原24 h检测淋巴细胞线粒体DNA（mitochondrial DNA,mt DNA）中8-氧鸟嘌呤脱氧核苷（8-oxo-deoxyguanosine,8-oxod G）含量、线粒体复合体活性、8-氧鸟嘌呤DNA糖基化酶（8-oxoguanine DNA glycosylase-1,OGG1）蛋白表达。【结果】与NT组比较,HIT组淋巴细胞OGG1蛋白表达、线粒体复合体I、II、IV和ATP合成酶活性显著升高（P〈0.05）,mt DNA中8-oxod G含量显著降低（P〈0.01）。【结论】平原阶段短期高强度间歇运动训练可提高机体高原低氧环境下淋巴细胞线粒体抗氧化能力和能量代谢水平。

英文摘要：

【Objective】To explore the effect of short-term high-intensity interval exercise training（HIT） in plain on rush entry into plateau induced mitochondrial DNA oxidative damage in leukocyte.【Methods】Thirty male lowlanders were randomly divided into two groups： untrained group（NT, n = 15） and HIT group（HIT, n = 15）. Participants in the HIT group run at a high training intensity, 90%~95% heart rate reserve（HRR） for 3 min, with active pauses of 3 min of walking at 45%~50% of HRR. Participants in the HIT group underwent six sessions of HIT for 36 min/day, 7 days per week for 2 weeks. After training, participants in both HIT and NT groups traveled into plateau in the same batch. Leukocyte OGG1 protein expression, 8-oxod G content in mitochondrial DNA（mt DNA）, and activities of mitochondrial complex were measured in plateau at 24 h after entry.【Results】As compared with NT group, OGG1 protein expression, complex I, II, IV and ATP synthase activities were significantly elevated（P〈0.05）, whereas 8-oxod G content in mt DNA was significantly decreased（P〈0.01）.【Conclusion】Short-term high-intensity interval exercise training in plain can elevated mitochondrial antioxidant capacity and energy metabolism in high altitude hypoxia environment.