中文摘要：

目的探讨鸟苷酸交换因子C3G（Crk SH3-domain-binding guanine nucleotide-releasing factor）在卵巢癌组织中的表达及在卵巢癌SKOV3细胞增殖中的作用。方法采用Western blot检测比较40例卵巢癌、8例卵巢良性肿瘤及4例正常卵巢组织中C3G的表达水平。应用特异性siRNA下调SKOV3细胞中C3G的表达,通过MTT法和平板克隆实验检测细胞增殖,建立裸鼠皮下种植瘤模型观察移植瘤生长情况。结果卵巢癌组织中C3G的表达显著高于卵巢良性肿瘤及正常卵巢组织,其在卵巢癌、卵巢良性肿瘤分别是正常卵巢组织中的（1.832±0.200）（P〈0.05）、（0.893±0.147）倍。siRNA干扰可使SKOV3细胞中内源性C3G的表达下调79.2%;与野生型细胞组、阴性对照组及空载体对照组相比,C3G干扰组细胞增殖变慢（P〈0.05）,克隆形成率下降（P〈0.05）,移植瘤的平均体积和质量显著下降（P〈0.05）。结论鸟苷酸交换因子C3G在大多数上皮性卵巢癌中过表达,并促进卵巢癌细胞增殖。

英文摘要：

Objective To determine the expression of guanine nueleotide exchange factor Crk SH3- domain-binding guanine nucleotide-releasing factor （C3G） in epithelial ovarian cancer tissues and its role in the proliferation of human ovarian cancer cell line SKOV3. Methods C3G was detected by Western blotting in the tissue samples of epithelial ovarian cancer （ n = 40）, benign ovarian tumors （ n = 8 ） and normal ovarian （n =4）, respectively. Endogenous C3G was knocked-down by siRNA in SKOV3 cells. Then, MTT assay and plate colony formation assay were used to evaluate the proliferation of cancer cells. Nude mice subcutaneous xenograft transplanted tumor model was established to assess tumor growth in vivo. Results The expression of C3G was significantly higher in epithelial ovarian cancer tissues than in benign ovarian tumors and normal ovari- an tissues. The expression of C3G was 1. 832 ±0. 200 times higher in epithelial ovarian cancer tissues（ P 〈 0. 05 ） and 0.893 ±0. 147 times higher in benign tumors when compared with normal ovarian tissues, respec- tively. Specific siRNA knocked down resulted in the expression of C3G decreased by 79.2% in SKOV3 cells, and significant decrease in cell proliferation and clone formation rate （P 〈 0. 05 ）. Nude mice xenograft trans- planted tumor by C3G siRNA transfected SKOV3 cells had average smaller volume and lower weight of sub- cutaneous tumors （P 〈0. 05）. Conclusion Nucleotide exchange factor C3G, over-expressed in most epithelial ovarian cancer tissues, facilitates the proliferation of ovarian cancer SKOV3 cells.