中文摘要：

目的：探讨胃癌患者血清mi R-486的表达水平及其辅助诊断的价值。方法：收集经病理确诊的100例胃癌患者、30例胃息肉患者及50例健康对照者的血清标本。采用荧光定量PCR方法定量检测血清mi R-486的表达水平,用化学发光法检测CEA、CAl99、CA724的含量。采用Mann-Whitney U检验比较不同组间血清mi R-486水平的差异,采用χ2检验分析胃癌患者血清mi R-486水平与其临床病理特征之间的关系。采用Spearman相关性分析胃癌患者血清mi R-486水平与CEA、CA199及CA724的关系,应用ROC曲线及AUC（95%CI）评估它们作为诊断指标的诊断效能。结果：胃癌、胃息肉患者和健康对照者血清mi R-486的含量分别为0.454（0.225,0.653）、0.121（0.083,0.166）和0.063（0.013,0.119）;胃癌患者血清mi R-486的含量显著高于胃息肉患者和健康对照者,差异均具有统计学意义（P均〈0.001）;而胃息肉患者与健康对照者血清mi R-486的含量差异无统计学意义（P〉0.05）。胃癌患者血清mi R-486的表达水平在不同年龄、性别、胃癌部位无统计学差异（P〉0.05）,在分化程度（P=0.046）、TNM分期（P=0.013）、淋巴结是否转移（P=0.006）差异均有统计学意义。胃癌患者血清mi R-486含量与CEA（r=0.213,P=0.034）、CA724（r=0.297,P=0.003）有相关性,与CA199（r=0.037,P=0.714）无相关性。胃癌组与健康对照组比较,血清mi R-486的AUC 0.839,95%CI为0.774~0.904。结论：胃癌患者血清mi R-486含量显著高于胃息肉患者和健康对照者,mi R-486可能是胃癌辅助诊断的一个重要生物学指标。

英文摘要：

Objective：To investigate the expression of mi R-486 in serum of patients with gastric cancer and its value to auxiliary diagnosis. Methods： The serum of 100 patients with pathological confirmed GC, 30 gastric polyps and 50 healthy controls were recruited. Serum mi R-486 expression was determined by fluorescent quantity PCR. Serum CEA, CA199 and CA724 were determined by chemiluminescence method. Comparison of differences of serum mi R-486 between groups was performed by Mann-Whitney U test.Analysis the relationship between serum expression of mi R-486 in gastric cancer patients and its clinical pathological characteristics was performed by χ2test. The relationships between mi R-486 and CEA, CA199, CA724 were examined by Spearman correlation analysis. ROC curve and AUC（95% CI） were used to evaluate their diagnosis efficiency. Results： The relative expression of mi R-486 in GC, gastric polyps, healthy controls were 0.454（0.225, 0.653）, 0.121（0.083, 0.166）, 0.063（0.013, 0.119）,respectively. The relative expression of mi R-486 in GC was significantly higher than that in gastric polyps and healthy controls（P 〈 0.001）. The difference between gastric polyps and healthy controls is not significant（P 〉 0.05）. The differences of expression of mi R-486 in serum of GC in tumor differentiation（P=0.046）, TNM（P=0.013） and the occurrence of lymph node metastases（P=0.006）were statistically significant except for age, gender, tumor area（P 〉 0.05）. The relative expression of mi R-486 in GC had a correlation with CEA（r=0.213, P=0.034）,CA724（r=0.297, P=0.003） but no obvious correlation with CA199（r=0.037, P=0.714）. AUC of serum mi R-486 was 0.839, 0.774~0.904（95%CI） in discriminating GC from healthy controls. Conclusion： The relative expression of mi R-486 in GC was significantly higher than that in gastric polyps and healthy controls, serum mi R-486 may be a biomarker for the auxiliary diagnosis of GC.