中文摘要：

开发了两步亲和色谱法：肝素-琼脂糖凝胶、Ni-琼脂糖凝胶色谱纯化人血浆中硒蛋白-P的方法,并采用氢化物发生-原子荧光分光光度法（HG-AFS）检测,成功搭建了硒蛋白-P的纯化检测平台。确定了亲和色谱纯化的最佳梯度洗脱条件,通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）定性检测,得到了一定纯度的硒蛋白-P,其回收率达43.2%。HG-AFS方法的线性相关系数为0.999 1,检出限为0.09μg/L,日内精密度（RSD）为0.12%,日间精密度（RSD）为0.27%,加标回收率为95%～104%。该亲和色谱纯化方法简单易控、回收率高,HG-AFS检测灵敏度高,结果准确可靠。

英文摘要：

A method was developed for the purification and detection of selenoprotein - P in human plasma. The sample was gradient eluted by two successive steps of affinity chromatography, i.e. , Heparin - Sepharose and Ni - Sepharose chromatography. The quality of the purified selenoprotein - P was detected by HG - AFS. The optimum gradient elution conditions were determined and the sele- noprotein - P with a certain purity was obtained by SDS - PAGE. The recovery of the two steps of af- finity chromatography reached up to 43.2%. The correlation coefficient of HG - AFS was 0. 999 1, and the detection limit was 0. 09 μg/L. The RSDs were 0. 12% for within-day and 0.27% for inter- day. The recoveries were in the range of 95% - 104% . The proposed method was simple and sen- sitive, and the results .obtained were accurate.