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羧酸富勒烯C3对K562和AHH-1细胞的辐射生物效应研究
  • ISSN号:0254-5098
  • 期刊名称:《中华放射医学与防护杂志》
  • 时间:0
  • 分类:R733.7[医药卫生—肿瘤;医药卫生—临床医学] R14[医药卫生—公共卫生与预防医学]
  • 作者机构:[1]第二军医大学放射医学研究室,上海200433
  • 相关基金:国家自然科学基金(30570552);全军医药卫生“十一五”攻关课题(06G60)
中文摘要:

目的探讨羧酸富勒烯C3(C3)在辐射防护和辅助放疗方面的应用。方法以不同浓度C3与AHH-1、K562细胞共孵育,用CCK-8法、锥虫蓝试验检测C3对细胞活力和存活率的影响,用Annexin—V/PI染色、流式细胞分析法研究照射后细胞凋亡和细胞周期的变化。结果C3对AHH-1细胞活力几乎无影响(存活率大于95%),但600mg/L的C3使K562细胞存活率明显降低(82%)。100mg/LC3用药照射组与单纯照射组相比,4Gy照射后AHH-1细胞的存活率显著升高(71.3%、90.3%),细胞凋亡率明显下降(26.3%、12.6%);而受照射24Gy的K562细胞存活率却呈现下降趋势(69.4%、66.1%),不同剂量照射后细胞凋亡率反而增加。细胞周期分析结果显示,C3处理组AHH-1细胞12Gy辐射后G2期阻滞(27.2%)与单纯照射组(40.8%)相比减轻;而K562受照细胞则反而加重。结论C3对AHH-1细胞具有较好的辐射防护作用,而对K562细胞则表现为抑制细胞增殖,促进辐射诱导的细胞凋亡并加重G2期阻滞。

英文摘要:

Objective To investigate the application prospective of carboxyfullerene C3 as a radioprotectant or assistant for tumor radiotherapy. Methods Different concentrations of C3 were incubated with K562 and AHH-1 cell, CCK-8 assay and trypan blue rejection test were performed to examine the influence of C3 on the cell viability. AnnexinV/Pl staining and flow cytometry assay were applied to assess the cell cycle and apoptosis after γ-ray irradiation. Results C3 showed little toxicity to AHH-1 cell with the survival rate over 95% , but 600 mg/L of C3 markedly inhibited the growth of K562 ce11(82% ). Pretreatment of 100 mg/L C3 significantly increased the survival rate of AHH-1 cell after 4 Gy irradiation compared with the single radiation group(71.3% vs 90. 3% ) , but decreased the apoptosis rate (26. 3% vs 12.6% ), while the survival rate of K562 cell was decreased and the apoptosis rate was elevated with the increase of C3 concentration. Moreover, the cell cycle analysis revealed the G2 phase block in AHH-1 cell after radiation exposure was mitigated by C3 pretreatment, but that in K562 cell was aggravated. Conclusions C3 has good radioprotective effects on AHH-1 cells. For K562 cell, C3 could inhibit the cell proliferation, promote the radiation induced apoptosis and aggravate the G2 phase block.

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期刊信息
  • 《中华放射医学与防护杂志》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学技术协会
  • 主办单位:中华医学会
  • 主编:
  • 地址:北京市德外新康街2号
  • 邮编:100088
  • 邮箱:cjrmp@cjrmp.sina.net
  • 电话:010-62389620
  • 国际标准刊号:ISSN:0254-5098
  • 国内统一刊号:ISSN:11-2271/R
  • 邮发代号:18-93
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:11741